Diagnostic Molecular Mycobacteriology in Regions With Low Tuberculosis Endemicity: Combining Real-time PCR Assays for Detection of Multiple Mycobacterial Pathogens With Line Probe Assays for Identification of Resistance Mutations

Diagnostic Molecular Mycobacteriology in Regions With Low Tuberculosis Endemicity: Combining Real-time PCR Assays for Detection of Multiple Mycobacterial Pathogens With Line Probe Assays for Identification of Resistance Mutations

Molecular assays have not yet been able to replace time-consuming culture-based methods in clinical mycobacteriology. Using 6875 clinical samples and a study period of 35 months we evaluated the use of PCR-based assays to establish a diagnostic workflow with a fast time-to-result of 1–2 days, for 1....

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Bibliographic Details
Main Authors: Vanessa Deggim-Messmer, Guido V. Bloemberg, Claudia Ritter, Antje Voit, Rico Hömke, Peter M. Keller, Erik C. Böttger
Format: Article
Language:English
Published: Elsevier 2016-07-01
Series:EBioMedicine
Subjects:
Real-time PCR
Line probe assay
Mycobacterium tuberculosis
Nontuberculous mycobacteria
Drug susceptibility
Online Access:http://www.sciencedirect.com/science/article/pii/S2352396416302614

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http://www.sciencedirect.com/science/article/pii/S2352396416302614

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