Long-Term Culture of Glutamine Synthetase-Transfected HepG2 Cells in Circulatory Flow Bioreactor for Development of a Bioartificial Liver

Long-Term Culture of Glutamine Synthetase-Transfected HepG2 Cells in Circulatory Flow Bioreactor for Development of a Bioartificial Liver

Glutamine synthetase (GS) is involved in an accessory pathway of ammonia removal in mammals. To develop a bioartificial liver with a human cell line, GS gene was transfected into HepG2 cells, which had no ammonia removal activity. After culturing in the presence of methionine sulfoximine (MSX), a GS...

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Bibliographic Details
Main Authors: Shin Enosawa, Tomoyuki Miyashita, Seiichi Suzuki, Xiao-Kang Li, Miyuki Tsunoda, Hiroshi Amemiya, Mitsugu Yamanaka, Shinya Hiramatsu, Naoko Tanimura, Takeshi Omasa, Kenichi Suga, Toshiharu Matsumura
Format: Article
Language:English
Published: SAGE Publishing 2000-09-01
Series:Cell Transplantation
Online Access:https://doi.org/10.1177/096368970000900520

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https://doi.org/10.1177/096368970000900520

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