NMR Structure Determinations of Small Proteins Using only One Fractionally 20% <sup>13</sup>C- and Uniformly 100% <sup>15</sup>N-Labeled Sample

NMR Structure Determinations of Small Proteins Using only One Fractionally 20% <sup>13</sup>C- and Uniformly 100% <sup>15</sup>N-Labeled Sample

Uniformly <sup>13</sup>C- and <sup>15</sup>N-labeled samples ensure fast and reliable nuclear magnetic resonance (NMR) assignments of proteins and are commonly used for structure elucidation by NMR. However, the preparation of uniformly labeled samples is a labor-intensive an...

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Bibliographic Details
Main Authors: Harri A. Heikkinen, Sofia M. Backlund, Hideo Iwaï
Format: Article
Language:English
Published: MDPI AG 2021-02-01
Series:Molecules
Subjects:
NMR structure determination
fractional <sup>13</sup>C-labeling
biosynthetically directed fractional <sup>13</sup>C-labeling
NMR
biosynthetic pathways
protein structure
Online Access:https://www.mdpi.com/1420-3049/26/3/747

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https://www.mdpi.com/1420-3049/26/3/747

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