Glutaraldehyde Cross-Linking of Immobilized Thermophilic Esterase on Hydrophobic Macroporous Resin for Application in Poly(ε-caprolactone) Synthesis

Glutaraldehyde Cross-Linking of Immobilized Thermophilic Esterase on Hydrophobic Macroporous Resin for Application in Poly(ε-caprolactone) Synthesis

The immobilized thermophilic esterase from Archaeoglobus fulgidus was successfully constructed through the glutaraldehyde-mediated covalent coupling after its physical adsorption on a hydrophobic macroporous resin, Sepabeads EC-OD. Through 0.05% glutaraldehyde treatment, the prevention of enzyme lea...

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Bibliographic Details
Main Authors: Min Wang, Hui Shi, Di Wu, Haobo Han, Jianxu Zhang, Zhen Xing, Shuang Wang, Quanshun Li
Format: Article
Language:English
Published: MDPI AG 2014-07-01
Series:Molecules
Subjects:
immobilized enzyme
cross linking
thermophilic esterase
ring-opening polymerization
ε-caprolactone
Online Access:http://www.mdpi.com/1420-3049/19/7/9838

Internet

http://www.mdpi.com/1420-3049/19/7/9838

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