Rapid identification of invasive fungal species using sensitive universal primers-based PCR and restriction endonuclease digestions coupled with high-resolution melting analysis

Backgound: Conventional diagnosis of invasive fungal disease from blood cultures is often notoriously delayed and inadequately sensitive. We aimed to develop a universal primers-based polymerase chain reaction (PCR) assay and restriction fragment length polymorphisms (RFLP) for rapid identification...

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Bibliographic Details
Main Authors: Ming-Horng Tsai, Lee-Chung Lin, Jen-Fu Hsu, Mei-Yin Lai, Hsuan-Rong Huang, Ming-Chou Chiang, Jang-Jih Lu
Format: Article
Language:English
Published: Elsevier 2019-10-01
Series:Journal of Microbiology, Immunology and Infection
Online Access:http://www.sciencedirect.com/science/article/pii/S1684118218305012