A protocol for qualitative and quantitative measurement of endosomal processing using hot spot analysis

A protocol for qualitative and quantitative measurement of endosomal processing using hot spot analysis

Summary: A detailed quantification of antigen processing by endosomal compartments provides important information on the pattern of protein fragmentation. Here, we describe a protocol that combines gradient purified endosomes, incubated with antigens, followed by hot spot analysis of MS/MS-sequenced...

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Bibliographic Details
Main Authors: Cristina C. Clement, Padma P. Nanaware, Lawrence J. Stern, Laura Santambrogio
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
cell biology
cell separation/fractionation
immunology
mass spectrometry
protein biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721003555

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http://www.sciencedirect.com/science/article/pii/S2666166721003555

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