Lentiviral vectors and protocols for creation of stable hESC lines for fluorescent tracking and drug resistance selection of cardiomyocytes.

Developmental, physiological and tissue engineering studies critical to the development of successful myocardial regeneration therapies require new ways to effectively visualize and isolate large numbers of fluorescently labeled, functional cardiomyocytes.Here we describe methods for the clonal expa...

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Bibliographic Details
Main Authors: Hiroko Kita-Matsuo, Maria Barcova, Natalie Prigozhina, Nathan Salomonis, Karen Wei, Jeffrey G Jacot, Brandon Nelson, Sean Spiering, René Haverslag, Changsung Kim, Maria Talantova, Ruchi Bajpai, Diego Calzolari, Alexey Terskikh, Andrew D McCulloch, Jeffrey H Price, Bruce R Conklin, H S Vincent Chen, Mark Mercola
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2009-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2662416?pdf=render