Identification of Interactions in the NMD Complex Using Proximity-Dependent Biotinylation (BioID).

Identification of Interactions in the NMD Complex Using Proximity-Dependent Biotinylation (BioID).

Proximity-dependent trans-biotinylation by the Escherichia coli biotin ligase BirA mutant R118G (BirA*) allows stringent streptavidin affinity purification of proximal proteins. This so-called BioID method provides an alternative to the widely used co-immunoprecipitation (co-IP) to identify protein-...

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Bibliographic Details
Main Authors: Christoph Schweingruber, Paolo Soffientini, Marc-David Ruepp, Angela Bachi, Oliver Mühlemann
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4774922?pdf=render

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http://europepmc.org/articles/PMC4774922?pdf=render

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