Capture of microRNA-bound mRNAs identifies the tumor suppressor miR-34a as a regulator of growth factor signaling.

Capture of microRNA-bound mRNAs identifies the tumor suppressor miR-34a as a regulator of growth factor signaling.

A simple biochemical method to isolate mRNAs pulled down with a transfected, biotinylated microRNA was used to identify direct target genes of miR-34a, a tumor suppressor gene. The method reidentified most of the known miR-34a regulated genes expressed in K562 and HCT116 cancer cell lines. Transcrip...

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Bibliographic Details
Main Authors: Ashish Lal, Marshall P Thomas, Gabriel Altschuler, Francisco Navarro, Elizabeth O'Day, Xiao Ling Li, Carla Concepcion, Yoon-Chi Han, Jerome Thiery, Danielle K Rajani, Aaron Deutsch, Oliver Hofmann, Andrea Ventura, Winston Hide, Judy Lieberman
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-11-01
Series:PLoS Genetics
Online Access:http://europepmc.org/articles/PMC3213160?pdf=render

Internet

http://europepmc.org/articles/PMC3213160?pdf=render

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