Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny.
The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have employed a sensitive reporter-based approach to demonst...
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doaj-c36bdc75aa094263aeb4f2c1eb9541052020-11-25T02:12:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01712e5136110.1371/journal.pone.0051361Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny.Simon D J CalaminusAmelie V GuitartAmy SinclairHannah SchachtnerSteve P WatsonTessa L HolyoakeKamil R KrancLaura M MacheskyThe development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have employed a sensitive reporter-based approach to demonstrate that Pf4-Cre also recombines in a significant proportion of both fetal liver and bone marrow hematopoietic stem cells (HSCs), including the most primitive fraction containing the long-term repopulating HSCs. Consequently, we demonstrate that Pf4-Cre activity is not megakaryocyte lineage-specific but extends to other myeloid and lymphoid lineages at significant levels between 15-60%. Finally, we show for the first time that Pf4 transcripts are present in adult HSCs and primitive hematopoietic progenitor cells. These results have fundamental implications for the use of the Pf4-Cre mouse model and for our understanding of a possible role for Pf4 in the development of the hematopoietic lineage.http://europepmc.org/articles/PMC3531453?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Simon D J Calaminus Amelie V Guitart Amy Sinclair Hannah Schachtner Steve P Watson Tessa L Holyoake Kamil R Kranc Laura M Machesky |
spellingShingle |
Simon D J Calaminus Amelie V Guitart Amy Sinclair Hannah Schachtner Steve P Watson Tessa L Holyoake Kamil R Kranc Laura M Machesky Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. PLoS ONE |
author_facet |
Simon D J Calaminus Amelie V Guitart Amy Sinclair Hannah Schachtner Steve P Watson Tessa L Holyoake Kamil R Kranc Laura M Machesky |
author_sort |
Simon D J Calaminus |
title |
Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. |
title_short |
Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. |
title_full |
Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. |
title_fullStr |
Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. |
title_full_unstemmed |
Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. |
title_sort |
lineage tracing of pf4-cre marks hematopoietic stem cells and their progeny. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have employed a sensitive reporter-based approach to demonstrate that Pf4-Cre also recombines in a significant proportion of both fetal liver and bone marrow hematopoietic stem cells (HSCs), including the most primitive fraction containing the long-term repopulating HSCs. Consequently, we demonstrate that Pf4-Cre activity is not megakaryocyte lineage-specific but extends to other myeloid and lymphoid lineages at significant levels between 15-60%. Finally, we show for the first time that Pf4 transcripts are present in adult HSCs and primitive hematopoietic progenitor cells. These results have fundamental implications for the use of the Pf4-Cre mouse model and for our understanding of a possible role for Pf4 in the development of the hematopoietic lineage. |
url |
http://europepmc.org/articles/PMC3531453?pdf=render |
work_keys_str_mv |
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