Mapping a leaf senescence gene els1 by BSR-Seq in common wheat

Leaf senescence is normally the last stage of plant development. Early senescence of functional leaves significantly reduces the photosynthetic time and efficiency, seriously affecting grain yield and quality in wheat. Discovering genes responsible for early leaf senescence (els) are necessary for d...

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Main Authors:	Miaomiao Li, Beibei Li, Guanghao Guo, Yongxing Chen, Jingzhong Xie, Ping Lu, Qiuhong Wu, Deyun Zhang, Huaizhi Zhang, Jian Yang, Panpan Zhang, Yan Zhang, Zhiyong Liu
Format:	Article
Language:	English
Published:	KeAi Communications Co., Ltd. 2018-06-01
Series:	Crop Journal
Online Access:	http://www.sciencedirect.com/science/article/pii/S2214514118300199

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spelling	doaj-c3d832289d634161927904841d169f4a2021-04-02T13:18:23ZengKeAi Communications Co., Ltd.Crop Journal2214-51412018-06-0163236243Mapping a leaf senescence gene els1 by BSR-Seq in common wheatMiaomiao Li0Beibei Li1Guanghao Guo2Yongxing Chen3Jingzhong Xie4Ping Lu5Qiuhong Wu6Deyun Zhang7Huaizhi Zhang8Jian Yang9Panpan Zhang10Yan Zhang11Zhiyong Liu12College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, ChinaCollege of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaCollege of Horticulture, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; Corresponding author.Leaf senescence is normally the last stage of plant development. Early senescence of functional leaves significantly reduces the photosynthetic time and efficiency, seriously affecting grain yield and quality in wheat. Discovering genes responsible for early leaf senescence (els) are necessary for developing novel germplasms and cultivars with delayed leaf-senescence through molecular manipulation and marker assisted selection. In this study, we identified an early leaf senescence line M114 in a derivative of a wheat breeding population. Genetic analysis indicated that early leaf senescence in M114 is controlled by a single recessive gene, provisionally designated els1. By applying bulked segregant analysis and RNA-Seq (BSR-Seq), seven polymorphic markers linked to els1 were developed and the gene was located on chromosome arm 2BS in a 1.5 cM genetic interval between markers WGGB303 and WGGB305. A co-segregating marker, WGGB302, provide a starting point for fine mapping and map-based cloning of els1. Keywords: Bulk segregant analysis, Genetic mapping, Leaf senescence, Triticum aestivumhttp://www.sciencedirect.com/science/article/pii/S2214514118300199
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Miaomiao Li Beibei Li Guanghao Guo Yongxing Chen Jingzhong Xie Ping Lu Qiuhong Wu Deyun Zhang Huaizhi Zhang Jian Yang Panpan Zhang Yan Zhang Zhiyong Liu
spellingShingle	Miaomiao Li Beibei Li Guanghao Guo Yongxing Chen Jingzhong Xie Ping Lu Qiuhong Wu Deyun Zhang Huaizhi Zhang Jian Yang Panpan Zhang Yan Zhang Zhiyong Liu Mapping a leaf senescence gene els1 by BSR-Seq in common wheat Crop Journal
author_facet	Miaomiao Li Beibei Li Guanghao Guo Yongxing Chen Jingzhong Xie Ping Lu Qiuhong Wu Deyun Zhang Huaizhi Zhang Jian Yang Panpan Zhang Yan Zhang Zhiyong Liu
author_sort	Miaomiao Li
title	Mapping a leaf senescence gene els1 by BSR-Seq in common wheat
title_short	Mapping a leaf senescence gene els1 by BSR-Seq in common wheat
title_full	Mapping a leaf senescence gene els1 by BSR-Seq in common wheat
title_fullStr	Mapping a leaf senescence gene els1 by BSR-Seq in common wheat
title_full_unstemmed	Mapping a leaf senescence gene els1 by BSR-Seq in common wheat
title_sort	mapping a leaf senescence gene els1 by bsr-seq in common wheat
publisher	KeAi Communications Co., Ltd.
series	Crop Journal
issn	2214-5141
publishDate	2018-06-01
description	Leaf senescence is normally the last stage of plant development. Early senescence of functional leaves significantly reduces the photosynthetic time and efficiency, seriously affecting grain yield and quality in wheat. Discovering genes responsible for early leaf senescence (els) are necessary for developing novel germplasms and cultivars with delayed leaf-senescence through molecular manipulation and marker assisted selection. In this study, we identified an early leaf senescence line M114 in a derivative of a wheat breeding population. Genetic analysis indicated that early leaf senescence in M114 is controlled by a single recessive gene, provisionally designated els1. By applying bulked segregant analysis and RNA-Seq (BSR-Seq), seven polymorphic markers linked to els1 were developed and the gene was located on chromosome arm 2BS in a 1.5 cM genetic interval between markers WGGB303 and WGGB305. A co-segregating marker, WGGB302, provide a starting point for fine mapping and map-based cloning of els1. Keywords: Bulk segregant analysis, Genetic mapping, Leaf senescence, Triticum aestivum
url	http://www.sciencedirect.com/science/article/pii/S2214514118300199
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Mapping a leaf senescence gene els1 by BSR-Seq in common wheat

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