Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress
Abstract ACE2 and TMPRSS2 are key players on SARS-CoV-2 entry into host cells. However, it is still unclear whether expression levels of these factors could reflect disease severity. Here, a case–control study was conducted with 213 SARS-CoV-2 positive individuals where cases were defined as COVID-1...
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Nature Publishing Group
2021-05-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-021-88944-8 |
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Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Átila Duque Rossi João Locke Ferreira de Araújo Tailah Bernardo de Almeida Marcelo Ribeiro-Alves Camila de Almeida Velozo Jéssica Maciel de Almeida Isabela de Carvalho Leitão Sâmila Natiane Ferreira Jéssica da Silva Oliveira Hugo José Alves Helena Toledo Scheid Débora Souza Faffe Rafael Mello Galliez Renata Eliane de Ávila Gustavo Gomes Resende Mauro Martins Teixeira COVID-19 UFRJ Workgroup Orlando da Costa Ferreira Júnior Terezinha Marta P. P. Castiñeiras Renan Pedra Souza Amilcar Tanuri Renato Santana de Aguiar Shana Priscila Coutinho Barroso Cynthia Chester Cardoso |
spellingShingle |
Átila Duque Rossi João Locke Ferreira de Araújo Tailah Bernardo de Almeida Marcelo Ribeiro-Alves Camila de Almeida Velozo Jéssica Maciel de Almeida Isabela de Carvalho Leitão Sâmila Natiane Ferreira Jéssica da Silva Oliveira Hugo José Alves Helena Toledo Scheid Débora Souza Faffe Rafael Mello Galliez Renata Eliane de Ávila Gustavo Gomes Resende Mauro Martins Teixeira COVID-19 UFRJ Workgroup Orlando da Costa Ferreira Júnior Terezinha Marta P. P. Castiñeiras Renan Pedra Souza Amilcar Tanuri Renato Santana de Aguiar Shana Priscila Coutinho Barroso Cynthia Chester Cardoso Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress Scientific Reports |
author_facet |
Átila Duque Rossi João Locke Ferreira de Araújo Tailah Bernardo de Almeida Marcelo Ribeiro-Alves Camila de Almeida Velozo Jéssica Maciel de Almeida Isabela de Carvalho Leitão Sâmila Natiane Ferreira Jéssica da Silva Oliveira Hugo José Alves Helena Toledo Scheid Débora Souza Faffe Rafael Mello Galliez Renata Eliane de Ávila Gustavo Gomes Resende Mauro Martins Teixeira COVID-19 UFRJ Workgroup Orlando da Costa Ferreira Júnior Terezinha Marta P. P. Castiñeiras Renan Pedra Souza Amilcar Tanuri Renato Santana de Aguiar Shana Priscila Coutinho Barroso Cynthia Chester Cardoso |
author_sort |
Átila Duque Rossi |
title |
Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress |
title_short |
Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress |
title_full |
Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress |
title_fullStr |
Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress |
title_full_unstemmed |
Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress |
title_sort |
association between ace2 and tmprss2 nasopharyngeal expression and covid-19 respiratory distress |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-05-01 |
description |
Abstract ACE2 and TMPRSS2 are key players on SARS-CoV-2 entry into host cells. However, it is still unclear whether expression levels of these factors could reflect disease severity. Here, a case–control study was conducted with 213 SARS-CoV-2 positive individuals where cases were defined as COVID-19 patients with respiratory distress requiring oxygen support (N = 38) and controls were those with mild to moderate symptoms of the disease who did not need oxygen therapy along the entire clinical course (N = 175). ACE2 and TMPRSS2 mRNA levels were evaluated in nasopharyngeal swab samples by RT-qPCR and logistic regression analyzes were applied to estimate associations with respiratory outcomes. ACE2 and TMPRSS2 levels positively correlated with age, which was also strongly associated with respiratory distress. Increased nasopharyngeal ACE2 levels showed a protective effect against this outcome (adjOR = 0.30; 95% CI 0.09–0.91), while TMPRSS2/ACE2 ratio was associated with risk (adjOR = 4.28; 95% CI 1.36–13.48). On stepwise regression, TMPRSS2/ACE2 ratio outperformed ACE2 to model COVID-19 severity. When nasopharyngeal swabs were compared to bronchoalveolar lavages in an independent cohort of COVID-19 patients under mechanical ventilation, similar expression levels of these genes were observed. These data suggest nasopharyngeal TMPRSS2/ACE2 as a promising candidate for further prediction models on COVID-19. |
url |
https://doi.org/10.1038/s41598-021-88944-8 |
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doaj-c427b492385a4d0f84ccc25eea32d6722021-05-09T11:34:18ZengNature Publishing GroupScientific Reports2045-23222021-05-011111910.1038/s41598-021-88944-8Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distressÁtila Duque Rossi0João Locke Ferreira de Araújo1Tailah Bernardo de Almeida2Marcelo Ribeiro-Alves3Camila de Almeida Velozo4Jéssica Maciel de Almeida5Isabela de Carvalho Leitão6Sâmila Natiane Ferreira7Jéssica da Silva Oliveira8Hugo José Alves9Helena Toledo Scheid10Débora Souza Faffe11Rafael Mello Galliez12Renata Eliane de Ávila13Gustavo Gomes Resende14Mauro Martins Teixeira15COVID-19 UFRJ WorkgroupOrlando da Costa Ferreira Júnior16Terezinha Marta P. P. Castiñeiras17Renan Pedra Souza18Amilcar Tanuri19Renato Santana de Aguiar20Shana Priscila Coutinho Barroso21Cynthia Chester Cardoso22Laboratório de Virologia Molecular, Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de JaneiroLaboratório de Biologia Integrativa, Departamento de Genética, Ecologia e Evolução, Instituto de Ciências Biológicas, Universidade Federal de Minas GeraisInstituto de Estudos do Mar Almirante Paulo Moreira, Marinha do BrasilInstituto Nacional de Infectologia Evandro Chagas, FiocruzLaboratório de Virologia Molecular, Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de JaneiroLaboratório de Virologia Molecular, Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de JaneiroInstituto de Biofísica, Universidade Federal do Rio de JaneiroInstituto de Pesquisas Biomédicas, Hospital Naval Marcílio Dias, Marinha do BrasilInstituto de Pesquisas Biomédicas, Hospital Naval Marcílio Dias, Marinha do BrasilLaboratório de Biologia Integrativa, Departamento de Genética, Ecologia e Evolução, Instituto de Ciências Biológicas, Universidade Federal de Minas GeraisFaculdade de Medicina, Universidade Federal do Rio de JaneiroInstituto de Biofísica, Universidade Federal do Rio de JaneiroFaculdade de Medicina, Universidade Federal do Rio de JaneiroHospital Eduardo de MenezesHospital das Clínicas, Universidade Federal de Minas Gerais (HC-UFMG/EBSERH)Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas GeraisLaboratório de Virologia Molecular, Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de JaneiroFaculdade de Medicina, Universidade Federal do Rio de JaneiroLaboratório de Biologia Integrativa, Departamento de Genética, Ecologia e Evolução, Instituto de Ciências Biológicas, Universidade Federal de Minas GeraisLaboratório de Virologia Molecular, Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de JaneiroLaboratório de Biologia Integrativa, Departamento de Genética, Ecologia e Evolução, Instituto de Ciências Biológicas, Universidade Federal de Minas GeraisInstituto de Pesquisas Biomédicas, Hospital Naval Marcílio Dias, Marinha do BrasilLaboratório de Virologia Molecular, Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de JaneiroAbstract ACE2 and TMPRSS2 are key players on SARS-CoV-2 entry into host cells. However, it is still unclear whether expression levels of these factors could reflect disease severity. Here, a case–control study was conducted with 213 SARS-CoV-2 positive individuals where cases were defined as COVID-19 patients with respiratory distress requiring oxygen support (N = 38) and controls were those with mild to moderate symptoms of the disease who did not need oxygen therapy along the entire clinical course (N = 175). ACE2 and TMPRSS2 mRNA levels were evaluated in nasopharyngeal swab samples by RT-qPCR and logistic regression analyzes were applied to estimate associations with respiratory outcomes. ACE2 and TMPRSS2 levels positively correlated with age, which was also strongly associated with respiratory distress. Increased nasopharyngeal ACE2 levels showed a protective effect against this outcome (adjOR = 0.30; 95% CI 0.09–0.91), while TMPRSS2/ACE2 ratio was associated with risk (adjOR = 4.28; 95% CI 1.36–13.48). On stepwise regression, TMPRSS2/ACE2 ratio outperformed ACE2 to model COVID-19 severity. When nasopharyngeal swabs were compared to bronchoalveolar lavages in an independent cohort of COVID-19 patients under mechanical ventilation, similar expression levels of these genes were observed. These data suggest nasopharyngeal TMPRSS2/ACE2 as a promising candidate for further prediction models on COVID-19.https://doi.org/10.1038/s41598-021-88944-8 |