High-quality ultrastructural preservation using cryofixation for 3D electron microscopy of genetically labeled tissues

High-quality ultrastructural preservation using cryofixation for 3D electron microscopy of genetically labeled tissues

Electron microscopy (EM) offers unparalleled power to study cell substructures at the nanoscale. Cryofixation by high-pressure freezing offers optimal morphological preservation, as it captures cellular structures instantaneously in their near-native state. However, the applicability of cryofixation...

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Bibliographic Details
Main Authors: Tin Ki Tsang, Eric A Bushong, Daniela Boassa, Junru Hu, Benedetto Romoli, Sebastien Phan, Davide Dulcis, Chih-Ying Su, Mark H Ellisman
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2018-05-01
Series:eLife
Subjects:
cryofixation by high pressure freezing
APEX2
CryoChem
genetic labeling
correlated light and electron microscopy
serial block-face scanning electron microscopy
Online Access:https://elifesciences.org/articles/35524

Internet

https://elifesciences.org/articles/35524

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