Transport of gallocatechin gallate and catechin gallate in high-temperature-processed green tea extract from gastrointestinal tract to brain by an in vitro bio-mimic model system coupled with sequential cell cultures

• Main Authors: Kang-Hyun Jeong, Si-Young Cho, Yong-Deog Hong, Jin-Oh Chung, Kwang-Sik Kim, Soon-Mi Shim
• Format: Article
• Language: English
• Published: Elsevier 2018-08-01
• Series: Journal of Functional Foods
• Subjects: GCG; CG; Metabolite; In vitro bio-mimic model
• Online Access: http://www.sciencedirect.com/science/article/pii/S1756464618302457
• ISSN: 1756-4646

The aim of this study was to evaluate the transport of gallocatechin gallate (GCG), catechin gallate (CG) and their metabolites in high-temperature-processed green tea extract (HTP_GTE) from the gastrointestinal tract to blood-brain barrier (BBB) with an in vitro bio-mimic model system with sequential cell cultures including Caco-2, HepG2 and HBMECs. Transports from the GI to BBB, the final concentrations of GCC and CG were 18.95 ± 3.24 μM and 5.11 ± 0.83 μM, respectively. Metabolites detected in BBB after systemic circulation were identified as glucuronide or sulfate conjugated form. Results suggest that GCG, CG and their metabolites in HTP_GTE are capable of reaching the brain by oral intake of HTP_GTE, implying that HTP_GTE could be utilized as a natural functional material for the prevention of degenerative brain diseases.