A Universal, Genomewide GuideFinder for CRISPR/Cas9 Targeting in Microbial Genomes

With the explosion in our understanding of human and environmental microbial diversity, corresponding efforts to understand gene function in these organisms are strongly needed. CRISPR/Cas9 technology has revolutionized interrogation of gene function in a wide variety of model organisms. Efficient C...

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Bibliographic Details
Main Authors: Michelle Spoto, Changhui Guan, Elizabeth Fleming, Julia Oh
Format: Article
Language:English
Published: American Society for Microbiology 2020-02-01
Series:mSphere
Subjects:
Online Access:https://doi.org/10.1128/mSphere.00086-20