Preparation, Characterization, and Biological Evaluation of Poly(Glutamic Acid)-b-Polyphenylalanine Polymersomes
Different types of amphiphilic macromolecular structures have been developed within recent decades to prepare the polymer particles considered as drug delivery systems. In the present research the series of amphiphilic block-copolymers containing poly(glutamatic acid) as hydrophilic, and polyphenyla...
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doaj-c4aa95931c9c4b8ca78a496ff1a4c3982020-11-24T21:41:20ZengMDPI AGPolymers2073-43602016-06-018621210.3390/polym8060212polym8060212Preparation, Characterization, and Biological Evaluation of Poly(Glutamic Acid)-b-Polyphenylalanine PolymersomesEvgenia Vlakh0Anastasiia Ananyan1Natalia Zashikhina2Anastasiia Hubina3Aleksander Pogodaev4Mariia Volokitina5Vladimir Sharoyko6Tatiana Tennikova7Institute of Chemistry, Saint-Petersburg State University, Universitesky pr. 26, 198504 St. Petersburg, RussiaInstitute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy pr. 31, 199004 St. Petersburg, RussiaInstitute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy pr. 31, 199004 St. Petersburg, RussiaInstitute of Chemistry, Saint-Petersburg State University, Universitesky pr. 26, 198504 St. Petersburg, RussiaInstitute of Chemistry, Saint-Petersburg State University, Universitesky pr. 26, 198504 St. Petersburg, RussiaInstitute of Chemistry, Saint-Petersburg State University, Universitesky pr. 26, 198504 St. Petersburg, RussiaInstitute of Chemistry, Saint-Petersburg State University, Universitesky pr. 26, 198504 St. Petersburg, RussiaInstitute of Chemistry, Saint-Petersburg State University, Universitesky pr. 26, 198504 St. Petersburg, RussiaDifferent types of amphiphilic macromolecular structures have been developed within recent decades to prepare the polymer particles considered as drug delivery systems. In the present research the series of amphiphilic block-copolymers containing poly(glutamatic acid) as hydrophilic, and polyphenylalanine as hydrophobic blocks was synthesized and characterized. Molecular weights for homo- and copolymers were determined by gel-permeation chromatography (GPC) and amino acid analysis, respectively. The copolymers obtained were applied for preparation of polymer particles. The specific morphology of prepared polymerosomes was proved using transmission electron microscopy (TEM). The influence on particle size of polymer concentration and pH used for self-assembly, as well as on the length of hydrophobic and hydrophilic blocks of applied copolymers, was studied by dynamic light scattering (DLS). Depending on different experimental conditions, the formation of nanoparticles with sizes from 60 to 350 nm was observed. The surface of polymersomes was modified with model protein (enzyme). No loss in biocatalytic activity was detected. Additionally, the process of encapsulation of model dyes was developed and the possibility of intracellular delivery of the dye-loaded nanoparticles was proved. Thus, the nanoparticles discussed can be considered for the creation of modern drug delivery systems.http://www.mdpi.com/2073-4360/8/6/212poly(amino acid)samphiphilic copolymerspolymer particlespolymersomesencapsulationbiodegradationcell uptake |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Evgenia Vlakh Anastasiia Ananyan Natalia Zashikhina Anastasiia Hubina Aleksander Pogodaev Mariia Volokitina Vladimir Sharoyko Tatiana Tennikova |
spellingShingle |
Evgenia Vlakh Anastasiia Ananyan Natalia Zashikhina Anastasiia Hubina Aleksander Pogodaev Mariia Volokitina Vladimir Sharoyko Tatiana Tennikova Preparation, Characterization, and Biological Evaluation of Poly(Glutamic Acid)-b-Polyphenylalanine Polymersomes Polymers poly(amino acid)s amphiphilic copolymers polymer particles polymersomes encapsulation biodegradation cell uptake |
author_facet |
Evgenia Vlakh Anastasiia Ananyan Natalia Zashikhina Anastasiia Hubina Aleksander Pogodaev Mariia Volokitina Vladimir Sharoyko Tatiana Tennikova |
author_sort |
Evgenia Vlakh |
title |
Preparation, Characterization, and Biological Evaluation of Poly(Glutamic Acid)-b-Polyphenylalanine Polymersomes |
title_short |
Preparation, Characterization, and Biological Evaluation of Poly(Glutamic Acid)-b-Polyphenylalanine Polymersomes |
title_full |
Preparation, Characterization, and Biological Evaluation of Poly(Glutamic Acid)-b-Polyphenylalanine Polymersomes |
title_fullStr |
Preparation, Characterization, and Biological Evaluation of Poly(Glutamic Acid)-b-Polyphenylalanine Polymersomes |
title_full_unstemmed |
Preparation, Characterization, and Biological Evaluation of Poly(Glutamic Acid)-b-Polyphenylalanine Polymersomes |
title_sort |
preparation, characterization, and biological evaluation of poly(glutamic acid)-b-polyphenylalanine polymersomes |
publisher |
MDPI AG |
series |
Polymers |
issn |
2073-4360 |
publishDate |
2016-06-01 |
description |
Different types of amphiphilic macromolecular structures have been developed within recent decades to prepare the polymer particles considered as drug delivery systems. In the present research the series of amphiphilic block-copolymers containing poly(glutamatic acid) as hydrophilic, and polyphenylalanine as hydrophobic blocks was synthesized and characterized. Molecular weights for homo- and copolymers were determined by gel-permeation chromatography (GPC) and amino acid analysis, respectively. The copolymers obtained were applied for preparation of polymer particles. The specific morphology of prepared polymerosomes was proved using transmission electron microscopy (TEM). The influence on particle size of polymer concentration and pH used for self-assembly, as well as on the length of hydrophobic and hydrophilic blocks of applied copolymers, was studied by dynamic light scattering (DLS). Depending on different experimental conditions, the formation of nanoparticles with sizes from 60 to 350 nm was observed. The surface of polymersomes was modified with model protein (enzyme). No loss in biocatalytic activity was detected. Additionally, the process of encapsulation of model dyes was developed and the possibility of intracellular delivery of the dye-loaded nanoparticles was proved. Thus, the nanoparticles discussed can be considered for the creation of modern drug delivery systems. |
topic |
poly(amino acid)s amphiphilic copolymers polymer particles polymersomes encapsulation biodegradation cell uptake |
url |
http://www.mdpi.com/2073-4360/8/6/212 |
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