Synthetic antibodies against BRIL as universal fiducial marks for single−particle cryoEM structure determination of membrane proteins

Synthetic antibodies against BRIL as universal fiducial marks for single−particle cryoEM structure determination of membrane proteins

Single particle cryo-electron microscopy of membrane proteins is limited by their small size and difficulty to orient. Here the authors generate recombinant antibodies against the 12 kDa fusion partner BRIL domain from apocytochrome b562 to use them as plug and play fiducial marks for structure dete...

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Bibliographic Details
Main Authors: Somnath Mukherjee, Satchal K. Erramilli, Mark Ammirati, Frances J. D. Alvarez, Kimberly F. Fennell, Michael D. Purdy, Blazej M. Skrobek, Katarzyna Radziwon, John Coukos, Yanyong Kang, Przemysław Dutka, Xiang Gao, Xiayang Qiu, Mark Yeager, H. Eric Xu, Seungil Han, Anthony A. Kossiakoff
Format: Article
Language:English
Published: Nature Publishing Group 2020-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-15363-0

Internet

https://doi.org/10.1038/s41467-020-15363-0

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