Identification, mapping and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time Fingerprinting
Mapping multiple glycan species to the same peptide and location from shared retention time of glycopeptides with MS and HPLC, Chalk, Greenland et al. construct a detailed mass-retention time database for Spike protein glycopeptides. This allows any mass LC-MS laboratory to reliably identify and qua...
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2021-08-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-021-02455-w |
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doaj-c526a34029cf4619809766a2928666022021-08-08T11:11:42ZengNature Publishing GroupCommunications Biology2399-36422021-08-01411810.1038/s42003-021-02455-wIdentification, mapping and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time FingerprintingRod Chalk0William E. P. Greenland1Tiago Moreira2Jesse Coker3Shubhashish M. M. Mukhopadhyay4Eleanor Williams5Charlotte Manning6Tina Bohstedt7Rama McCrorie8Alejandra Fernandez-Cid9Nicola A. Burgess-Brown10Centre for Medicines Discovery, ORCRB, University of OxfordAgilent Technologies, Lakeside, Cheadle Royal Business Park, CheadleCentre for Medicines Discovery, ORCRB, University of OxfordCentre for Medicines Discovery, ORCRB, University of OxfordCentre for Medicines Discovery, ORCRB, University of OxfordCentre for Medicines Discovery, ORCRB, University of OxfordCentre for Medicines Discovery, ORCRB, University of OxfordCentre for Medicines Discovery, ORCRB, University of OxfordCentre for Medicines Discovery, ORCRB, University of OxfordCentre for Medicines Discovery, ORCRB, University of OxfordCentre for Medicines Discovery, ORCRB, University of OxfordMapping multiple glycan species to the same peptide and location from shared retention time of glycopeptides with MS and HPLC, Chalk, Greenland et al. construct a detailed mass-retention time database for Spike protein glycopeptides. This allows any mass LC-MS laboratory to reliably identify and quantify Spike protein glycopeptides from a single overnight elastase protein digest in less than 90 minutes.https://doi.org/10.1038/s42003-021-02455-w |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Rod Chalk William E. P. Greenland Tiago Moreira Jesse Coker Shubhashish M. M. Mukhopadhyay Eleanor Williams Charlotte Manning Tina Bohstedt Rama McCrorie Alejandra Fernandez-Cid Nicola A. Burgess-Brown |
| spellingShingle |
Rod Chalk William E. P. Greenland Tiago Moreira Jesse Coker Shubhashish M. M. Mukhopadhyay Eleanor Williams Charlotte Manning Tina Bohstedt Rama McCrorie Alejandra Fernandez-Cid Nicola A. Burgess-Brown Identification, mapping and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time Fingerprinting Communications Biology |
| author_facet |
Rod Chalk William E. P. Greenland Tiago Moreira Jesse Coker Shubhashish M. M. Mukhopadhyay Eleanor Williams Charlotte Manning Tina Bohstedt Rama McCrorie Alejandra Fernandez-Cid Nicola A. Burgess-Brown |
| author_sort |
Rod Chalk |
| title |
Identification, mapping and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time Fingerprinting |
| title_short |
Identification, mapping and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time Fingerprinting |
| title_full |
Identification, mapping and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time Fingerprinting |
| title_fullStr |
Identification, mapping and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time Fingerprinting |
| title_full_unstemmed |
Identification, mapping and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time Fingerprinting |
| title_sort |
identification, mapping and relative quantitation of sars-cov-2 spike glycopeptides by mass-retention time fingerprinting |
| publisher |
Nature Publishing Group |
| series |
Communications Biology |
| issn |
2399-3642 |
| publishDate |
2021-08-01 |
| description |
Mapping multiple glycan species to the same peptide and location from shared retention time of glycopeptides with MS and HPLC, Chalk, Greenland et al. construct a detailed mass-retention time database for Spike protein glycopeptides. This allows any mass LC-MS laboratory to reliably identify and quantify Spike protein glycopeptides from a single overnight elastase protein digest in less than 90 minutes. |
| url |
https://doi.org/10.1038/s42003-021-02455-w |
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