A validated high-resolution accurate mass LC-MS assay for quantitative determination of metoprolol and α-hydroxymetoprolol in human serum for application in pharmacokinetics
To determine metoprolol and its metabolite α-hydroxymetoprolol in human serum we validated a method on an LC system with an Exactive® Orbitrap mass spectrometer (Thermo Scientific) as detector and isotope-labelled metoprolol-d7 as internal standard. A simple sample preparation was used with water-ac...
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Online Access: | https://doi.org/10.17145/jab.17.008 |
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doaj-c52ddcedd5164faf9803205269d4a29b2020-11-25T02:38:28ZengBetasciencepress PublishersJournal of Applied Bioanalysis2405-710X2017-06-0133495710.17145/jab.17.008A validated high-resolution accurate mass LC-MS assay for quantitative determination of metoprolol and α-hydroxymetoprolol in human serum for application in pharmacokineticsSjoukje Postma-Kunnen0Jan Peter Yska1Gerard Hommema2Sikke Koopmans3Bob Wilffert4Eric N. van Roon5Department of Clinical Pharmacy and Clinical Pharmacology, Medical Centre Leeuwarden, Leeuwarden, the NetherlandsDepartment of Clinical Pharmacy and Clinical Pharmacology, Medical Centre Leeuwarden, Leeuwarden, the NetherlandsDepartment of Clinical Pharmacy and Clinical Pharmacology, Medical Centre Leeuwarden, Leeuwarden, the NetherlandsDepartment of Clinical Pharmacy and Clinical Pharmacology, Medical Centre Leeuwarden, Leeuwarden, the NetherlandsUniversity of Groningen, Groningen Research Institute of Pharmacy, PharmacoTherapy, -Epidemiology & -Economics, Groningen, the Netherlands & Department of Clinical Pharmacy and Pharmacology, University Medical Center Groningen, University of Groningen, Groningen, the NetherlandsDepartment of Clinical Pharmacy and Clinical Pharmacology, Medical Centre Leeuwarden, Leeuwarden, the Netherlands & University of Groningen, Groningen Research Institute of Pharmacy, PharmacoTherapy, -Epidemiology & -Economics, Groningen, the NetherlandsTo determine metoprolol and its metabolite α-hydroxymetoprolol in human serum we validated a method on an LC system with an Exactive® Orbitrap mass spectrometer (Thermo Scientific) as detector and isotope-labelled metoprolol-d7 as internal standard. A simple sample preparation was used with water-acetonitrile (15:85, v/v) as precipitation reagent. This method has a chromatographic run time of 15 min and linear calibration curves in the range of 5.0-250 μg/L for both metoprolol and α-hydroxymetoprolol. Validation showed the method to be accurate, with a good precision, selective and with a lower limit of quantitation of 2.0 μg/L for metoprolol and 1.0 μg/L for α-hydroxymetoprolol, respectively. This validated LC-Orbitrap MS analysis for metoprolol and α-hydroxymetoprolol can be used for application in human pharmacokinetics.https://doi.org/10.17145/jab.17.008metoprololα-hydroxymetoprololLC-MSorbitrap mass spectrometerpharmacokinetics. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sjoukje Postma-Kunnen Jan Peter Yska Gerard Hommema Sikke Koopmans Bob Wilffert Eric N. van Roon |
spellingShingle |
Sjoukje Postma-Kunnen Jan Peter Yska Gerard Hommema Sikke Koopmans Bob Wilffert Eric N. van Roon A validated high-resolution accurate mass LC-MS assay for quantitative determination of metoprolol and α-hydroxymetoprolol in human serum for application in pharmacokinetics Journal of Applied Bioanalysis metoprolol α-hydroxymetoprolol LC-MS orbitrap mass spectrometer pharmacokinetics. |
author_facet |
Sjoukje Postma-Kunnen Jan Peter Yska Gerard Hommema Sikke Koopmans Bob Wilffert Eric N. van Roon |
author_sort |
Sjoukje Postma-Kunnen |
title |
A validated high-resolution accurate mass LC-MS assay for quantitative determination of metoprolol and α-hydroxymetoprolol in human serum for application in pharmacokinetics |
title_short |
A validated high-resolution accurate mass LC-MS assay for quantitative determination of metoprolol and α-hydroxymetoprolol in human serum for application in pharmacokinetics |
title_full |
A validated high-resolution accurate mass LC-MS assay for quantitative determination of metoprolol and α-hydroxymetoprolol in human serum for application in pharmacokinetics |
title_fullStr |
A validated high-resolution accurate mass LC-MS assay for quantitative determination of metoprolol and α-hydroxymetoprolol in human serum for application in pharmacokinetics |
title_full_unstemmed |
A validated high-resolution accurate mass LC-MS assay for quantitative determination of metoprolol and α-hydroxymetoprolol in human serum for application in pharmacokinetics |
title_sort |
validated high-resolution accurate mass lc-ms assay for quantitative determination of metoprolol and α-hydroxymetoprolol in human serum for application in pharmacokinetics |
publisher |
Betasciencepress Publishers |
series |
Journal of Applied Bioanalysis |
issn |
2405-710X |
publishDate |
2017-06-01 |
description |
To determine metoprolol and its metabolite α-hydroxymetoprolol in human serum we validated a method on an LC system with an Exactive® Orbitrap mass spectrometer (Thermo Scientific) as detector and isotope-labelled metoprolol-d7 as internal standard. A simple sample preparation was used with water-acetonitrile (15:85, v/v) as precipitation reagent. This method has a chromatographic run time of 15 min and linear calibration curves in the range of 5.0-250 μg/L for both metoprolol and α-hydroxymetoprolol. Validation showed the method to be accurate, with a good precision, selective and with a lower limit of quantitation of 2.0 μg/L for metoprolol and 1.0 μg/L for α-hydroxymetoprolol, respectively. This validated LC-Orbitrap MS analysis for metoprolol and α-hydroxymetoprolol can be used for application in human pharmacokinetics. |
topic |
metoprolol α-hydroxymetoprolol LC-MS orbitrap mass spectrometer pharmacokinetics. |
url |
https://doi.org/10.17145/jab.17.008 |
work_keys_str_mv |
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