WheatCRISPR: a web-based guide RNA design tool for CRISPR/Cas9-mediated genome editing in wheat

WheatCRISPR: a web-based guide RNA design tool for CRISPR/Cas9-mediated genome editing in wheat

Abstract Background CRISPR/Cas9 gene editing has become a revolutionary technique for crop improvement as it can facilitate fast and efficient genetic changes without the retention of transgene components in the final plant line. Lack of robust bioinformatics tools to facilitate the design of highly...

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Bibliographic Details
Main Authors: Dustin Cram, Manoj Kulkarni, Miles Buchwaldt, Nandhakishore Rajagopalan, Pankaj Bhowmik, Kevin Rozwadowski, Isobel A. P. Parkin, Andrew G. Sharpe, Sateesh Kagale
Format: Article
Language:English
Published: BMC 2019-11-01
Series:BMC Plant Biology
Subjects:
CRISPR
Cas9
gRNA design tool
Genome editing
Transcriptional regulation
Wheat
Online Access:http://link.springer.com/article/10.1186/s12870-019-2097-z

Internet

http://link.springer.com/article/10.1186/s12870-019-2097-z

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