Advantageous direct quantification of viable closely related probiotics in petit-suisse cheeses under in vitro gastrointestinal conditions by Propidium Monoazide--qPCR.

Advantageous direct quantification of viable closely related probiotics in petit-suisse cheeses under in vitro gastrointestinal conditions by Propidium Monoazide--qPCR.

Species-specific Quantitative Real Time PCR (qPCR) alone and combined with the use of propidium monoazide (PMA) were used along with the plate count method to evaluate the survival of the probiotic strains Lactobacillus acidophilus La-5 and Bifidobacterium animalis subsp. lactis Bb-12, and the bacte...

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Bibliographic Details
Main Authors: Martha Lissete Morales Villarreal, Marina Padilha, Antonio Diogo Silva Vieira, Bernadette Dora Gombossy de Melo Franco, Rafael Chacon Ruiz Martinez, Susana Marta Isay Saad
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3866109?pdf=render

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http://europepmc.org/articles/PMC3866109?pdf=render

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