De novo strategy with engineering anti-Kasha/Kasha fluorophores enables reliable ratiometric quantification of biomolecules

Fluorescent probes are used in a number of fields but suffer from a lack of quantifiable results due to environmental effects. Here, the authors report on a dual-emission probe which can be used to detect the amount of probe present and the emission from detection applications to allow for quantific...

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Bibliographic Details
Main Authors: Limin Shi, Chenxu Yan, Zhiqian Guo, Weijie Chi, Jingle Wei, Weimin Liu, Xiaogang Liu, He Tian, Wei-Hong Zhu
Format: Article
Language:English
Published: Nature Publishing Group 2020-02-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-14615-3