Examination of Exhaustive Cloning Attempts Reveals that C. elegans piRNAs, Transposons, and Repeat Sequences are Efficiently Cloned in Yeast, but not in Bacteria

Genome sequencing requires insertion of random fragments of the sequenced organism’s DNA into a unicellular host, most often E. coli bacteria. This manipulation was found in the past to be analogous to naturally occurring horizontal gene transfer, and moreover has proved valuable to understanding to...

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Bibliographic Details
Main Authors: Or eSagy, Ron eShamir, Oded eRechavi
Format: Article
Language:English
Published: Frontiers Media S.A. 2014-08-01
Series:Frontiers in Genetics
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fgene.2014.00275/full