An optimized protocol for the retroviral transduction of mouse CD4 T cells

An optimized protocol for the retroviral transduction of mouse CD4 T cells

Summary: Transduction of primary T cells has become prominent with the introduction of chimeric antigen receptor T-cell therapy. Although there are many protocols for the transduction of human T cells, it remains a challenge to transduce murine T cells. We present an optimized protocol for the retro...

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Bibliographic Details
Main Authors: Ekaterina Eremenko, Zoe V. Taylor, Bishnu Khand, Shir Zaccai, Angel Porgador, Alon Monsonego
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Cell culture
Cell-based Assays
Cell separation/fractionation
Immunology
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721004263

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http://www.sciencedirect.com/science/article/pii/S2666166721004263

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