An optimized protocol for the retroviral transduction of mouse CD4 T cells

Summary: Transduction of primary T cells has become prominent with the introduction of chimeric antigen receptor T-cell therapy. Although there are many protocols for the transduction of human T cells, it remains a challenge to transduce murine T cells. We present an optimized protocol for the retro...

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Main Authors:	Ekaterina Eremenko, Zoe V. Taylor, Bishnu Khand, Shir Zaccai, Angel Porgador, Alon Monsonego
Format:	Article
Language:	English
Published:	Elsevier 2021-09-01
Series:	STAR Protocols
Subjects:	Cell culture Cell-based Assays Cell separation/fractionation Immunology Molecular Biology
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166721004263

id	doaj-c64dd8db782a4cf282175d61248356e4
record_format	Article
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Ekaterina Eremenko Zoe V. Taylor Bishnu Khand Shir Zaccai Angel Porgador Alon Monsonego
spellingShingle	Ekaterina Eremenko Zoe V. Taylor Bishnu Khand Shir Zaccai Angel Porgador Alon Monsonego An optimized protocol for the retroviral transduction of mouse CD4 T cells STAR Protocols Cell culture Cell-based Assays Cell separation/fractionation Immunology Molecular Biology
author_facet	Ekaterina Eremenko Zoe V. Taylor Bishnu Khand Shir Zaccai Angel Porgador Alon Monsonego
author_sort	Ekaterina Eremenko
title	An optimized protocol for the retroviral transduction of mouse CD4 T cells
title_short	An optimized protocol for the retroviral transduction of mouse CD4 T cells
title_full	An optimized protocol for the retroviral transduction of mouse CD4 T cells
title_fullStr	An optimized protocol for the retroviral transduction of mouse CD4 T cells
title_full_unstemmed	An optimized protocol for the retroviral transduction of mouse CD4 T cells
title_sort	optimized protocol for the retroviral transduction of mouse cd4 t cells
publisher	Elsevier
series	STAR Protocols
issn	2666-1667
publishDate	2021-09-01
description	Summary: Transduction of primary T cells has become prominent with the introduction of chimeric antigen receptor T-cell therapy. Although there are many protocols for the transduction of human T cells, it remains a challenge to transduce murine T cells. We present an optimized protocol for the retroviral transduction of murine CD4 T cells, which overcomes major challenges including large-scale production and long-term culturing of transduced cells. The optimized protocol combines high transduction efficiency with a low rate of cell death.For complete details on the use and execution of this protocol, please refer to Eremenko et al., 2019.
topic	Cell culture Cell-based Assays Cell separation/fractionation Immunology Molecular Biology
url	http://www.sciencedirect.com/science/article/pii/S2666166721004263
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spelling	doaj-c64dd8db782a4cf282175d61248356e42021-09-19T05:00:45ZengElsevierSTAR Protocols2666-16672021-09-0123100719An optimized protocol for the retroviral transduction of mouse CD4 T cellsEkaterina Eremenko0Zoe V. Taylor1Bishnu Khand2Shir Zaccai3Angel Porgador4Alon Monsonego5The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The Department of Life Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The National Institute of Biotechnology in the Negev, Zlotowski Neuroscience Center, and Regenerative Medicine and Stem Cell Research Center, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; Corresponding authorThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The National Institute of Biotechnology in the Negev, Zlotowski Neuroscience Center, and Regenerative Medicine and Stem Cell Research Center, Ben-Gurion University of the Negev, Beer Sheva 8410501, IsraelThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The National Institute of Biotechnology in the Negev, Zlotowski Neuroscience Center, and Regenerative Medicine and Stem Cell Research Center, Ben-Gurion University of the Negev, Beer Sheva 8410501, IsraelThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The Department of Life Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The National Institute of Biotechnology in the Negev, Zlotowski Neuroscience Center, and Regenerative Medicine and Stem Cell Research Center, Ben-Gurion University of the Negev, Beer Sheva 8410501, IsraelThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The National Institute of Biotechnology in the Negev, Zlotowski Neuroscience Center, and Regenerative Medicine and Stem Cell Research Center, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; Corresponding authorThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The National Institute of Biotechnology in the Negev, Zlotowski Neuroscience Center, and Regenerative Medicine and Stem Cell Research Center, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; Corresponding authorSummary: Transduction of primary T cells has become prominent with the introduction of chimeric antigen receptor T-cell therapy. Although there are many protocols for the transduction of human T cells, it remains a challenge to transduce murine T cells. We present an optimized protocol for the retroviral transduction of murine CD4 T cells, which overcomes major challenges including large-scale production and long-term culturing of transduced cells. The optimized protocol combines high transduction efficiency with a low rate of cell death.For complete details on the use and execution of this protocol, please refer to Eremenko et al., 2019.http://www.sciencedirect.com/science/article/pii/S2666166721004263Cell cultureCell-based AssaysCell separation/fractionationImmunologyMolecular Biology

An optimized protocol for the retroviral transduction of mouse CD4 T cells

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