Development and validation of a real-time PCR assay for detection and quantification of <it>Tuber magnatum</it> in soil

Development and validation of a real-time PCR assay for detection and quantification of <it>Tuber magnatum</it> in soil

<p>Abstract</p> <p>Background</p> <p><it>Tuber magnatum</it>, the Italian white truffle, is the most sought-after edible ectomycorrhizal mushroom. Previous studies report the difficulties of detecting its mycorrhizas and the widespread presence of its myceli...

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Bibliographic Details
Main Authors: Iotti Mirco, Leonardi Marco, Oddis Marilena, Salerni Elena, Baraldi Elena, Zambonelli Alessandra
Format: Article
Language:English
Published: BMC 2012-06-01
Series:BMC Microbiology
Subjects:
Real-time PCR
Taq-man probe
<it>Tuber magnatum</it> DNA concentration
Soil DNA extraction
ITS primers
Truffle production
Online Access:http://www.biomedcentral.com/1471-2180/12/93

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http://www.biomedcentral.com/1471-2180/12/93

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