Massive expansion and cryopreservation of functional human induced pluripotent stem cell-derived cardiomyocytes

Massive expansion and cryopreservation of functional human induced pluripotent stem cell-derived cardiomyocytes

Summary: Since the discovery of human induced pluripotent stem cells (hiPSCs), numerous strategies have been established to efficiently derive cardiomyocytes from hiPSCs (hiPSC-CMs). Here, we describe a cost-effective strategy for the subsequent massive expansion (>250-fold) of high-purity hiPSC-...

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Bibliographic Details
Main Authors: Renee G.C. Maas, Soah Lee, Magdalena Harakalova, Christian J.B. Snijders Blok, William R. Goodyer, Jesper Hjortnaes, Pieter A.F.M. Doevendans, Linda W. Van Laake, Jolanda van der Velden, Folkert W. Asselbergs, Joseph C. Wu, Joost P.G. Sluijter, Sean M. Wu, Jan W. Buikema
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Cell culture
Cell differentiation
Stem cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000411

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http://www.sciencedirect.com/science/article/pii/S2666166721000411

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