Changes in Posttraumatic Brain Edema in Craniectomy-Selective Brain Hypothermia Model Are Associated With Modulation of Aquaporin-4 Level
Both hypothermia and decompressive craniectomy have been considered as a treatment for traumatic brain injury. In previous experiments we established a murine model of decompressive craniectomy and we presented attenuated edema formation due to focal brain cooling. Since edema development is regulat...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2018-10-01
|
Series: | Frontiers in Neurology |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fneur.2018.00799/full |
id |
doaj-c68a3bf29fbe466aa3a801d5bedd04ad |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jacek Szczygielski Jacek Szczygielski Jacek Szczygielski Cosmin Glameanu Andreas Müller Markus Klotz Christoph Sippl Vanessa Hubertus Vanessa Hubertus Karl-Herbert Schäfer Angelika E. Mautes Karsten Schwerdtfeger Joachim Oertel |
spellingShingle |
Jacek Szczygielski Jacek Szczygielski Jacek Szczygielski Cosmin Glameanu Andreas Müller Markus Klotz Christoph Sippl Vanessa Hubertus Vanessa Hubertus Karl-Herbert Schäfer Angelika E. Mautes Karsten Schwerdtfeger Joachim Oertel Changes in Posttraumatic Brain Edema in Craniectomy-Selective Brain Hypothermia Model Are Associated With Modulation of Aquaporin-4 Level Frontiers in Neurology traumatic brain injury decompressive craniectomy brain edema hypothermia aquaporin-4 |
author_facet |
Jacek Szczygielski Jacek Szczygielski Jacek Szczygielski Cosmin Glameanu Andreas Müller Markus Klotz Christoph Sippl Vanessa Hubertus Vanessa Hubertus Karl-Herbert Schäfer Angelika E. Mautes Karsten Schwerdtfeger Joachim Oertel |
author_sort |
Jacek Szczygielski |
title |
Changes in Posttraumatic Brain Edema in Craniectomy-Selective Brain Hypothermia Model Are Associated With Modulation of Aquaporin-4 Level |
title_short |
Changes in Posttraumatic Brain Edema in Craniectomy-Selective Brain Hypothermia Model Are Associated With Modulation of Aquaporin-4 Level |
title_full |
Changes in Posttraumatic Brain Edema in Craniectomy-Selective Brain Hypothermia Model Are Associated With Modulation of Aquaporin-4 Level |
title_fullStr |
Changes in Posttraumatic Brain Edema in Craniectomy-Selective Brain Hypothermia Model Are Associated With Modulation of Aquaporin-4 Level |
title_full_unstemmed |
Changes in Posttraumatic Brain Edema in Craniectomy-Selective Brain Hypothermia Model Are Associated With Modulation of Aquaporin-4 Level |
title_sort |
changes in posttraumatic brain edema in craniectomy-selective brain hypothermia model are associated with modulation of aquaporin-4 level |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Neurology |
issn |
1664-2295 |
publishDate |
2018-10-01 |
description |
Both hypothermia and decompressive craniectomy have been considered as a treatment for traumatic brain injury. In previous experiments we established a murine model of decompressive craniectomy and we presented attenuated edema formation due to focal brain cooling. Since edema development is regulated via function of water channel proteins, our hypothesis was that the effects of decompressive craniectomy and of hypothermia are associated with a change in aquaporin-4 (AQP4) concentration. Male CD-1 mice were assigned into following groups (n = 5): sham, decompressive craniectomy, trauma, trauma followed by decompressive craniectomy and trauma + decompressive craniectomy followed by focal hypothermia. After 24 h, magnetic resonance imaging with volumetric evaluation of edema and contusion were performed, followed by ELISA analysis of AQP4 concentration in brain homogenates. Additional histopathological analysis of AQP4 immunoreactivity has been performed at more remote time point of 28d. Correlation analysis revealed a relationship between AQP4 level and both volume of edema (r2 = 0.45, p < 0.01, **) and contusion (r2 = 0.41, p < 0.01, **) 24 h after injury. Aggregated analysis of AQP4 level (mean ± SEM) presented increased AQP4 concentration in animals subjected to trauma and decompressive craniectomy (52.1 ± 5.2 pg/mL, p = 0.01; *), but not to trauma, decompressive craniectomy and hypothermia (45.3 ± 3.6 pg/mL, p > 0.05; ns) as compared with animals subjected to decompressive craniectomy only (32.8 ± 2.4 pg/mL). However, semiquantitative histopathological analysis at remote time point revealed no significant difference in AQP4 immunoreactivity across the experimental groups. This suggests that AQP4 is involved in early stages of brain edema formation after surgical decompression. The protective effect of selective brain cooling may be related to change in AQP4 response after decompressive craniectomy. The therapeutic potential of this interaction should be further explored. |
topic |
traumatic brain injury decompressive craniectomy brain edema hypothermia aquaporin-4 |
url |
https://www.frontiersin.org/article/10.3389/fneur.2018.00799/full |
work_keys_str_mv |
AT jacekszczygielski changesinposttraumaticbrainedemaincraniectomyselectivebrainhypothermiamodelareassociatedwithmodulationofaquaporin4level AT jacekszczygielski changesinposttraumaticbrainedemaincraniectomyselectivebrainhypothermiamodelareassociatedwithmodulationofaquaporin4level AT jacekszczygielski changesinposttraumaticbrainedemaincraniectomyselectivebrainhypothermiamodelareassociatedwithmodulationofaquaporin4level AT cosminglameanu changesinposttraumaticbrainedemaincraniectomyselectivebrainhypothermiamodelareassociatedwithmodulationofaquaporin4level AT andreasmuller changesinposttraumaticbrainedemaincraniectomyselectivebrainhypothermiamodelareassociatedwithmodulationofaquaporin4level AT markusklotz changesinposttraumaticbrainedemaincraniectomyselectivebrainhypothermiamodelareassociatedwithmodulationofaquaporin4level AT christophsippl changesinposttraumaticbrainedemaincraniectomyselectivebrainhypothermiamodelareassociatedwithmodulationofaquaporin4level AT vanessahubertus changesinposttraumaticbrainedemaincraniectomyselectivebrainhypothermiamodelareassociatedwithmodulationofaquaporin4level AT vanessahubertus changesinposttraumaticbrainedemaincraniectomyselectivebrainhypothermiamodelareassociatedwithmodulationofaquaporin4level AT karlherbertschafer changesinposttraumaticbrainedemaincraniectomyselectivebrainhypothermiamodelareassociatedwithmodulationofaquaporin4level AT angelikaemautes changesinposttraumaticbrainedemaincraniectomyselectivebrainhypothermiamodelareassociatedwithmodulationofaquaporin4level AT karstenschwerdtfeger changesinposttraumaticbrainedemaincraniectomyselectivebrainhypothermiamodelareassociatedwithmodulationofaquaporin4level AT joachimoertel changesinposttraumaticbrainedemaincraniectomyselectivebrainhypothermiamodelareassociatedwithmodulationofaquaporin4level |
_version_ |
1725045504280100864 |
spelling |
doaj-c68a3bf29fbe466aa3a801d5bedd04ad2020-11-25T01:40:29ZengFrontiers Media S.A.Frontiers in Neurology1664-22952018-10-01910.3389/fneur.2018.00799400932Changes in Posttraumatic Brain Edema in Craniectomy-Selective Brain Hypothermia Model Are Associated With Modulation of Aquaporin-4 LevelJacek Szczygielski0Jacek Szczygielski1Jacek Szczygielski2Cosmin Glameanu3Andreas Müller4Markus Klotz5Christoph Sippl6Vanessa Hubertus7Vanessa Hubertus8Karl-Herbert Schäfer9Angelika E. Mautes10Karsten Schwerdtfeger11Joachim Oertel12Department of Neurosurgery, Faculty of Medicine, Saarland University Medical Center, Saarland University, Homburg, GermanyInstitute of Neuropathology, Faculty of Medicine, Saarland University Medical Center, Saarland University, Homburg, GermanyFaculty of Medicine, University of Rzeszów, Rzeszów, PolandDepartment of Neurosurgery, Faculty of Medicine, Saarland University Medical Center, Saarland University, Homburg, GermanyDepartment of Radiology, Faculty of Medicine, Saarland University Medical Center, Saarland University, Homburg, GermanyWorking Group Enteric Nervous System (AGENS), University of Applied Sciences Kaiserslautern, Kaiserslautern, GermanyDepartment of Neurosurgery, Faculty of Medicine, Saarland University Medical Center, Saarland University, Homburg, GermanyDepartment of Neurosurgery, Faculty of Medicine, Saarland University Medical Center, Saarland University, Homburg, GermanyDepartment of Neurosurgery, Charité University Medicine, Berlin, GermanyWorking Group Enteric Nervous System (AGENS), University of Applied Sciences Kaiserslautern, Kaiserslautern, GermanyDepartment of Neurosurgery, Faculty of Medicine, Saarland University Medical Center, Saarland University, Homburg, GermanyDepartment of Neurosurgery, Faculty of Medicine, Saarland University Medical Center, Saarland University, Homburg, GermanyDepartment of Neurosurgery, Faculty of Medicine, Saarland University Medical Center, Saarland University, Homburg, GermanyBoth hypothermia and decompressive craniectomy have been considered as a treatment for traumatic brain injury. In previous experiments we established a murine model of decompressive craniectomy and we presented attenuated edema formation due to focal brain cooling. Since edema development is regulated via function of water channel proteins, our hypothesis was that the effects of decompressive craniectomy and of hypothermia are associated with a change in aquaporin-4 (AQP4) concentration. Male CD-1 mice were assigned into following groups (n = 5): sham, decompressive craniectomy, trauma, trauma followed by decompressive craniectomy and trauma + decompressive craniectomy followed by focal hypothermia. After 24 h, magnetic resonance imaging with volumetric evaluation of edema and contusion were performed, followed by ELISA analysis of AQP4 concentration in brain homogenates. Additional histopathological analysis of AQP4 immunoreactivity has been performed at more remote time point of 28d. Correlation analysis revealed a relationship between AQP4 level and both volume of edema (r2 = 0.45, p < 0.01, **) and contusion (r2 = 0.41, p < 0.01, **) 24 h after injury. Aggregated analysis of AQP4 level (mean ± SEM) presented increased AQP4 concentration in animals subjected to trauma and decompressive craniectomy (52.1 ± 5.2 pg/mL, p = 0.01; *), but not to trauma, decompressive craniectomy and hypothermia (45.3 ± 3.6 pg/mL, p > 0.05; ns) as compared with animals subjected to decompressive craniectomy only (32.8 ± 2.4 pg/mL). However, semiquantitative histopathological analysis at remote time point revealed no significant difference in AQP4 immunoreactivity across the experimental groups. This suggests that AQP4 is involved in early stages of brain edema formation after surgical decompression. The protective effect of selective brain cooling may be related to change in AQP4 response after decompressive craniectomy. The therapeutic potential of this interaction should be further explored.https://www.frontiersin.org/article/10.3389/fneur.2018.00799/fulltraumatic brain injurydecompressive craniectomybrain edemahypothermiaaquaporin-4 |