Correlation between resistance to trastuzumab and miR-141 relative expression in the BT-474 human breast cancer cell line
Background and Aim: Resistance to trastuzumab has been a critical barrier to targeted therapy of HER 2-positive (Human Epidermal Growth Factor Receptor 2) breast cancers. MicroRNAs (miRNAs) are known as decisive core regulators of drug resistance that modulate the epithelial-to-mesenchymal transitio...
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Birjand University of Medical Sciences and Health Services
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doaj-c69c4a4479c541aebebb523cf1e84d8d2020-11-24T21:43:00ZfasBirjand University of Medical Sciences and Health ServicesJournal of Birjand University of Medical Sciences1607-21972423-61522017-10-01243170178Correlation between resistance to trastuzumab and miR-141 relative expression in the BT-474 human breast cancer cell lineZohreh Rezaei0Dor Mohammad Kordi-Tamandani1Ahmadreza Sebzari2Kazem Dastjerdi3 Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand Cellular and Molecular Research Center, Birjand University of Medical Sciences , Birjnad Background and Aim: Resistance to trastuzumab has been a critical barrier to targeted therapy of HER 2-positive (Human Epidermal Growth Factor Receptor 2) breast cancers. MicroRNAs (miRNAs) are known as decisive core regulators of drug resistance that modulate the epithelial-to-mesenchymal transition (EMT) and cancer-related immune responses. The present study aimed at examining the expression of miR-141 in trastuzumab-resistant and trastuzumab-sensitive BT-474 breast cancer cells. Materials and Methods: In this experimental study, trastuzumab-resistant BT-474 cells were generated by continuous in-vitro culture of BT-474 cells in the presence of trastuzumab for six months. The relative expression of miR-141 to U6 RNA was then evaluated in trastuzumab-resistant and trastuzumab-sensitive cells using Relative Real-Time PCR. Mann-Whitney test was used to compare the difference between the two groups. Results: There was a significant difference between the survival rates of resistant BT-474 cells and sensitive cells in the MTT test in the presence of different concentrations of trastuzumab showing that BT-474 breast cancer cells have turned resistant to this drug under long-term culture (P=0.001). Also, the expression of miR-141 in trastuzumab-resistant cells was significantly reduced by four times compared with the BT-474 parent cells (P=0.049). Conclusion: Down-regulation of miR-141 in trastuzumab-resistant BT-474 cells might be one possible mechanism for resistance against trastuzumab and an indication of the role of this microRNA in controlling the metastasis pathway. http://journal.bums.ac.ir/browse.php?a_code=A-10-1232-1&slc_lang=en&sid=1Breast cancer erbB2/HER2 Trastuzumab resistance miR-141 |
collection |
DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
Zohreh Rezaei Dor Mohammad Kordi-Tamandani Ahmadreza Sebzari Kazem Dastjerdi |
spellingShingle |
Zohreh Rezaei Dor Mohammad Kordi-Tamandani Ahmadreza Sebzari Kazem Dastjerdi Correlation between resistance to trastuzumab and miR-141 relative expression in the BT-474 human breast cancer cell line Journal of Birjand University of Medical Sciences Breast cancer erbB2/HER2 Trastuzumab resistance miR-141 |
author_facet |
Zohreh Rezaei Dor Mohammad Kordi-Tamandani Ahmadreza Sebzari Kazem Dastjerdi |
author_sort |
Zohreh Rezaei |
title |
Correlation between resistance to trastuzumab and miR-141 relative expression in the BT-474 human breast cancer cell line |
title_short |
Correlation between resistance to trastuzumab and miR-141 relative expression in the BT-474 human breast cancer cell line |
title_full |
Correlation between resistance to trastuzumab and miR-141 relative expression in the BT-474 human breast cancer cell line |
title_fullStr |
Correlation between resistance to trastuzumab and miR-141 relative expression in the BT-474 human breast cancer cell line |
title_full_unstemmed |
Correlation between resistance to trastuzumab and miR-141 relative expression in the BT-474 human breast cancer cell line |
title_sort |
correlation between resistance to trastuzumab and mir-141 relative expression in the bt-474 human breast cancer cell line |
publisher |
Birjand University of Medical Sciences and Health Services |
series |
Journal of Birjand University of Medical Sciences |
issn |
1607-2197 2423-6152 |
publishDate |
2017-10-01 |
description |
Background and Aim: Resistance to trastuzumab has been a critical barrier to targeted therapy of HER 2-positive (Human Epidermal Growth Factor Receptor 2) breast cancers. MicroRNAs (miRNAs) are known as decisive core regulators of drug resistance that modulate the epithelial-to-mesenchymal transition (EMT) and cancer-related immune responses. The present study aimed at examining the expression of miR-141 in trastuzumab-resistant and trastuzumab-sensitive BT-474 breast cancer cells.
Materials and Methods: In this experimental study, trastuzumab-resistant BT-474 cells were generated by continuous in-vitro culture of BT-474 cells in the presence of trastuzumab for six months. The relative expression of miR-141 to U6 RNA was then evaluated in trastuzumab-resistant and trastuzumab-sensitive cells using Relative Real-Time PCR. Mann-Whitney test was used to compare the difference between the two groups.
Results: There was a significant difference between the survival rates of resistant BT-474 cells and sensitive cells in the MTT test in the presence of different concentrations of trastuzumab showing that BT-474 breast cancer cells have turned resistant to this drug under long-term culture (P=0.001). Also, the expression of miR-141 in trastuzumab-resistant cells was significantly reduced by four times compared with the BT-474 parent cells (P=0.049).
Conclusion: Down-regulation of miR-141 in trastuzumab-resistant BT-474 cells might be one possible mechanism for resistance against trastuzumab and an indication of the role of this microRNA in controlling the metastasis pathway.
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topic |
Breast cancer erbB2/HER2 Trastuzumab resistance miR-141 |
url |
http://journal.bums.ac.ir/browse.php?a_code=A-10-1232-1&slc_lang=en&sid=1 |
work_keys_str_mv |
AT zohrehrezaei correlationbetweenresistancetotrastuzumabandmir141relativeexpressioninthebt474humanbreastcancercellline AT dormohammadkorditamandani correlationbetweenresistancetotrastuzumabandmir141relativeexpressioninthebt474humanbreastcancercellline AT ahmadrezasebzari correlationbetweenresistancetotrastuzumabandmir141relativeexpressioninthebt474humanbreastcancercellline AT kazemdastjerdi correlationbetweenresistancetotrastuzumabandmir141relativeexpressioninthebt474humanbreastcancercellline |
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