Trypanosoma vivax: a simplified protocol for in vivo growth, isolation and cryopreservation

A rodent adapted clone of Trypanosoma vivax was used to infect cyclophosphamide treated mice and rats. Fresh blood containing trypanosomes, was centrifuged in a density gradient of three Percoll solutions, 1.07, 1.06, 1.05 g/ml, respectively, carefully layered on top of each other. The yields of thi...

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Bibliographic Details
Main Authors: Ndao M., Magnus E., Büscher P., Geerts S.
Format: Article
Language:English
Published: EDP Sciences 2004-03-01
Series:Parasite
Subjects:
Online Access:http://dx.doi.org/10.1051/parasite/2004111103