Trypanosoma vivax: a simplified protocol for in vivo growth, isolation and cryopreservation
A rodent adapted clone of Trypanosoma vivax was used to infect cyclophosphamide treated mice and rats. Fresh blood containing trypanosomes, was centrifuged in a density gradient of three Percoll solutions, 1.07, 1.06, 1.05 g/ml, respectively, carefully layered on top of each other. The yields of thi...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
EDP Sciences
2004-03-01
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Series: | Parasite |
Subjects: | |
Online Access: | http://dx.doi.org/10.1051/parasite/2004111103 |