Developmental Rewiring between Cerebellar Climbing Fibers and Purkinje Cells Begins with Positive Feedback Synapse Addition
Summary: During postnatal development, cerebellar climbing fibers alter their innervation strengths onto supernumerary Purkinje cell targets, generating a one-to-few connectivity pattern in adulthood. To get insight about the processes responsible for this remapping, we reconstructed serial electron...
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doaj-c6d6642ac7a04608b4f7a424f5b0a4c42020-11-25T01:32:27ZengElsevierCell Reports2211-12472019-11-0129928492861.e6Developmental Rewiring between Cerebellar Climbing Fibers and Purkinje Cells Begins with Positive Feedback Synapse AdditionAlyssa Michelle Wilson0Richard Schalek1Adi Suissa-Peleg2Thouis R. Jones3Seymour Knowles-Barley4Hanspeter Pfister5Jeff William Lichtman6Princeton Neuroscience Institute, Princeton University, Princeton, NJ 08540, USA; Corresponding authorCenter for Brain Science, Harvard University, Cambridge, MA 02138, USACenter for Brain Science, Harvard University, Cambridge, MA 02138, USA; School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USABroad Institute, Cambridge, MA 02142, USAVolpara Health Technologies, Wellington 6011, New ZealandSchool of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USADepartment of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA; Corresponding authorSummary: During postnatal development, cerebellar climbing fibers alter their innervation strengths onto supernumerary Purkinje cell targets, generating a one-to-few connectivity pattern in adulthood. To get insight about the processes responsible for this remapping, we reconstructed serial electron microscopy datasets from mice during the first postnatal week. Between days 3 and 7, individual climbing fibers selectively add many synapses onto a subset of Purkinje targets in a positive-feedback manner, without pruning synapses from other targets. Active zone sizes of synapses associated with powerful versus weak inputs are indistinguishable. Changes in synapse number are thus the predominant form of early developmental plasticity. Finally, the numbers of climbing fibers and Purkinje cells in a local region nearly match. Initial over-innervation of Purkinje cells by climbing fibers is therefore economical: the number of axons entering a region is enough to assure that each ultimately retains a postsynaptic target and that none branched there in vain. : Wilson et al. use electron microscopy to reveal that developmental rewiring in the cerebellum begins with significant synapse addition by climbing fibers onto a few preferred Purkinje cells. They also find that rewiring is economical: all climbing fibers initially entering a cerebellar region play a role in final connectivity there. Keywords: cerebellum, synapse elimination, climbing fibers, Purkinje cells, axonal convergence, axonal divergence, synapse formation, circuit development, connectomics, neuromuscular junctionhttp://www.sciencedirect.com/science/article/pii/S2211124719314032 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alyssa Michelle Wilson Richard Schalek Adi Suissa-Peleg Thouis R. Jones Seymour Knowles-Barley Hanspeter Pfister Jeff William Lichtman |
spellingShingle |
Alyssa Michelle Wilson Richard Schalek Adi Suissa-Peleg Thouis R. Jones Seymour Knowles-Barley Hanspeter Pfister Jeff William Lichtman Developmental Rewiring between Cerebellar Climbing Fibers and Purkinje Cells Begins with Positive Feedback Synapse Addition Cell Reports |
author_facet |
Alyssa Michelle Wilson Richard Schalek Adi Suissa-Peleg Thouis R. Jones Seymour Knowles-Barley Hanspeter Pfister Jeff William Lichtman |
author_sort |
Alyssa Michelle Wilson |
title |
Developmental Rewiring between Cerebellar Climbing Fibers and Purkinje Cells Begins with Positive Feedback Synapse Addition |
title_short |
Developmental Rewiring between Cerebellar Climbing Fibers and Purkinje Cells Begins with Positive Feedback Synapse Addition |
title_full |
Developmental Rewiring between Cerebellar Climbing Fibers and Purkinje Cells Begins with Positive Feedback Synapse Addition |
title_fullStr |
Developmental Rewiring between Cerebellar Climbing Fibers and Purkinje Cells Begins with Positive Feedback Synapse Addition |
title_full_unstemmed |
Developmental Rewiring between Cerebellar Climbing Fibers and Purkinje Cells Begins with Positive Feedback Synapse Addition |
title_sort |
developmental rewiring between cerebellar climbing fibers and purkinje cells begins with positive feedback synapse addition |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2019-11-01 |
description |
Summary: During postnatal development, cerebellar climbing fibers alter their innervation strengths onto supernumerary Purkinje cell targets, generating a one-to-few connectivity pattern in adulthood. To get insight about the processes responsible for this remapping, we reconstructed serial electron microscopy datasets from mice during the first postnatal week. Between days 3 and 7, individual climbing fibers selectively add many synapses onto a subset of Purkinje targets in a positive-feedback manner, without pruning synapses from other targets. Active zone sizes of synapses associated with powerful versus weak inputs are indistinguishable. Changes in synapse number are thus the predominant form of early developmental plasticity. Finally, the numbers of climbing fibers and Purkinje cells in a local region nearly match. Initial over-innervation of Purkinje cells by climbing fibers is therefore economical: the number of axons entering a region is enough to assure that each ultimately retains a postsynaptic target and that none branched there in vain. : Wilson et al. use electron microscopy to reveal that developmental rewiring in the cerebellum begins with significant synapse addition by climbing fibers onto a few preferred Purkinje cells. They also find that rewiring is economical: all climbing fibers initially entering a cerebellar region play a role in final connectivity there. Keywords: cerebellum, synapse elimination, climbing fibers, Purkinje cells, axonal convergence, axonal divergence, synapse formation, circuit development, connectomics, neuromuscular junction |
url |
http://www.sciencedirect.com/science/article/pii/S2211124719314032 |
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