Development and Application of a Label-Free Fluorescence Method for Determining the Composition of Gold Nanoparticle–Protein Conjugates

A method was developed for determining the composition of the conjugates between gold nanoparticles and proteins based on the intrinsic fluorescence of unbound protein molecules. The fluorescence was evaluated after separation of the conjugates from the reaction mixture by centrifugation. Gold nanop...

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Bibliographic Details
Main Authors: Dmitriy V. Sotnikov, Anatoly V. Zherdev, Boris B. Dzantiev
Format: Article
Language:English
Published: MDPI AG 2014-12-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:http://www.mdpi.com/1422-0067/16/1/907