Cell wall localization of the aspartic proteinase from buckwheat (FeAPL1) over-expressed in tobacco BY-2 cells

The recombinant aspartic proteinase-like protein (FeAPL1-His6) was overexpressed in the tobacco BY-2 cell line and the expected pepstatin A-sensitive enzymatic activity was confirmed at pH 3.0. Immunocytochemistry and protein gel blot analysis of the transformed BY-2 cells and their protoplasts show...

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Bibliographic Details
Main Authors: MIRA Đ. MILISAVLJEVIĆ, GORDANA S. TIMOTIJEVIĆ, DRAGANA B. NIKOLIĆ, JELENA T. SAMARDŽIĆ, VESNA R. MAKSIMOVIĆ
Format: Article
Language:English
Published: Serbian Chemical Society 2011-09-01
Series:Journal of the Serbian Chemical Society
Subjects:
Online Access:http://www.shd.org.rs/JSCS/Vol76/No9/05_4984_4199.pdf