UCE: A uracil excision (USER™)-based toolbox for transformation of cereals

<p>Abstract</p> <p>Background</p> <p>Cloning of gene casettes and other DNA sequences into the conventional vectors for biolistic or <it>Agrobacterium</it>-mediated transformation is hampered by a limited amount of unique restriction sites and by the difficu...

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Bibliographic Details
Main Authors: Brinch-Pedersen Henrik, Tauris Birgitte, Carciofi Massimiliano, Christiansen Michael W, Hebelstrup Kim H, Holm Preben B
Format: Article
Language:English
Published: BMC 2010-06-01
Series:Plant Methods
Online Access:http://www.plantmethods.com/content/6/1/15