Next Generation-Targeted Amplicon Sequencing (NG-TAS): an optimised protocol and computational pipeline for cost-effective profiling of circulating tumour DNA

Next Generation-Targeted Amplicon Sequencing (NG-TAS): an optimised protocol and computational pipeline for cost-effective profiling of circulating tumour DNA

Abstract Circulating tumour DNA (ctDNA) detection and monitoring have enormous potential clinical utility in oncology. We describe here a fast, flexible and cost-effective method to profile multiple genes simultaneously in low input cell-free DNA (cfDNA): Next Generation-Targeted Amplicon Sequencing...

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Bibliographic Details
Main Authors: Meiling Gao, Maurizio Callari, Emma Beddowes, Stephen-John Sammut, Marta Grzelak, Heather Biggs, Linda Jones, Abdelhamid Boumertit, Sabine C. Linn, Javier Cortes, Mafalda Oliveira, Richard Baird, Suet-Feung Chin, Carlos Caldas
Format: Article
Language:English
Published: BMC 2019-01-01
Series:Genome Medicine
Subjects:
NG-TAS
ctDNA
Liquid biopsy
Mutation
Multiplexing
Deep sequencing
Online Access:http://link.springer.com/article/10.1186/s13073-018-0611-9

Internet

http://link.springer.com/article/10.1186/s13073-018-0611-9

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