Identifying Anti-Oxidant Biosynthesis Genes in Pearl Millet [Pennisetum glaucum (L.) R. Br.] Using Genome—Wide Association Analysis
Pearl millet [Pennisetum glaucum (L.) R Br.] is an important staple food crop in the semi-arid tropics of Asia and Africa. It is a cereal grain that has the prospect to be used as a substitute for wheat flour for celiac patients. It is an important antioxidant food resource present with a wide range...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2021-05-01
|
Series: | Frontiers in Plant Science |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.599649/full |
id |
doaj-c7a89fb8272f42cb96e0536b3a77e348 |
---|---|
record_format |
Article |
spelling |
doaj-c7a89fb8272f42cb96e0536b3a77e3482021-05-28T09:44:47ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-05-011210.3389/fpls.2021.599649599649Identifying Anti-Oxidant Biosynthesis Genes in Pearl Millet [Pennisetum glaucum (L.) R. Br.] Using Genome—Wide Association AnalysisChandra Bhan Yadav0Jayanti Tokas1Jayanti Tokas2Devvart Yadav3Devvart Yadav4Ana Winters5Ram B. Singh6Rama Yadav7Prakash I. Gangashetty8Rakesh K. Srivastava9Rattan S. Yadav10Institute of Biological Environmental and Rural Sciences (IBERS), Aberystwyth University, Aberystwyth, United KingdomInstitute of Biological Environmental and Rural Sciences (IBERS), Aberystwyth University, Aberystwyth, United KingdomChaudhary Charan Singh (CCS) Haryana Agricultural University, Hisar, IndiaInstitute of Biological Environmental and Rural Sciences (IBERS), Aberystwyth University, Aberystwyth, United KingdomChaudhary Charan Singh (CCS) Haryana Agricultural University, Hisar, IndiaInstitute of Biological Environmental and Rural Sciences (IBERS), Aberystwyth University, Aberystwyth, United KingdomInternational Crops Research Institute for the Semi-Arid Tropics, Patancheru, IndiaInstitute of Biological Environmental and Rural Sciences (IBERS), Aberystwyth University, Aberystwyth, United KingdomInternational Crops Research Institute for the Semi-Arid Tropics, Niamey, NigerInternational Crops Research Institute for the Semi-Arid Tropics, Patancheru, IndiaInstitute of Biological Environmental and Rural Sciences (IBERS), Aberystwyth University, Aberystwyth, United KingdomPearl millet [Pennisetum glaucum (L.) R Br.] is an important staple food crop in the semi-arid tropics of Asia and Africa. It is a cereal grain that has the prospect to be used as a substitute for wheat flour for celiac patients. It is an important antioxidant food resource present with a wide range of phenolic compounds that are good sources of natural antioxidants. The present study aimed to identify the total antioxidant content of pearl millet flour and apply it to evaluate the antioxidant activity of its 222 genotypes drawn randomly from the pearl millet inbred germplasm association panel (PMiGAP), a world diversity panel of this crop. The total phenolic content (TPC) significantly correlated with DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity (% inhibition), which ranged from 2.32 to 112.45% and ferric-reducing antioxidant power (FRAP) activity ranging from 21.68 to 179.66 (mg ascorbic acid eq./100 g). Genome-wide association studies (GWAS) were conducted using 222 diverse accessions and 67 K SNPs distributed across all the seven pearl millet chromosomes. Approximately, 218 SNPs were found to be strongly associated with DPPH and FRAP activity at high confidence [–log (p) > 3.0–7.4]. Furthermore, flanking regions of significantly associated SNPs were explored for candidate gene harvesting. This identified 18 candidate genes related to antioxidant pathway genes (flavanone 7-O-beta-glycosyltransferase, GDSL esterase/lipase, glutathione S-transferase) residing within or near the association signal that can be selected for further functional characterization. Patterns of genetic variability and the associated genes reported in this study are useful findings, which would need further validation before their utilization in molecular breeding for high antioxidant-containing pearl millet cultivars.https://www.frontiersin.org/articles/10.3389/fpls.2021.599649/fullpearl millet [Pennisetum glaucum (L.) R. Br.]antioxidant activityphenolicsgermplasmgenome-wide association studymarker-trait associations |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chandra Bhan Yadav Jayanti Tokas Jayanti Tokas Devvart Yadav Devvart Yadav Ana Winters Ram B. Singh Rama Yadav Prakash I. Gangashetty Rakesh K. Srivastava Rattan S. Yadav |
spellingShingle |
Chandra Bhan Yadav Jayanti Tokas Jayanti Tokas Devvart Yadav Devvart Yadav Ana Winters Ram B. Singh Rama Yadav Prakash I. Gangashetty Rakesh K. Srivastava Rattan S. Yadav Identifying Anti-Oxidant Biosynthesis Genes in Pearl Millet [Pennisetum glaucum (L.) R. Br.] Using Genome—Wide Association Analysis Frontiers in Plant Science pearl millet [Pennisetum glaucum (L.) R. Br.] antioxidant activity phenolics germplasm genome-wide association study marker-trait associations |
author_facet |
Chandra Bhan Yadav Jayanti Tokas Jayanti Tokas Devvart Yadav Devvart Yadav Ana Winters Ram B. Singh Rama Yadav Prakash I. Gangashetty Rakesh K. Srivastava Rattan S. Yadav |
author_sort |
Chandra Bhan Yadav |
title |
Identifying Anti-Oxidant Biosynthesis Genes in Pearl Millet [Pennisetum glaucum (L.) R. Br.] Using Genome—Wide Association Analysis |
title_short |
Identifying Anti-Oxidant Biosynthesis Genes in Pearl Millet [Pennisetum glaucum (L.) R. Br.] Using Genome—Wide Association Analysis |
title_full |
Identifying Anti-Oxidant Biosynthesis Genes in Pearl Millet [Pennisetum glaucum (L.) R. Br.] Using Genome—Wide Association Analysis |
title_fullStr |
Identifying Anti-Oxidant Biosynthesis Genes in Pearl Millet [Pennisetum glaucum (L.) R. Br.] Using Genome—Wide Association Analysis |
title_full_unstemmed |
Identifying Anti-Oxidant Biosynthesis Genes in Pearl Millet [Pennisetum glaucum (L.) R. Br.] Using Genome—Wide Association Analysis |
title_sort |
identifying anti-oxidant biosynthesis genes in pearl millet [pennisetum glaucum (l.) r. br.] using genome—wide association analysis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2021-05-01 |
description |
Pearl millet [Pennisetum glaucum (L.) R Br.] is an important staple food crop in the semi-arid tropics of Asia and Africa. It is a cereal grain that has the prospect to be used as a substitute for wheat flour for celiac patients. It is an important antioxidant food resource present with a wide range of phenolic compounds that are good sources of natural antioxidants. The present study aimed to identify the total antioxidant content of pearl millet flour and apply it to evaluate the antioxidant activity of its 222 genotypes drawn randomly from the pearl millet inbred germplasm association panel (PMiGAP), a world diversity panel of this crop. The total phenolic content (TPC) significantly correlated with DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity (% inhibition), which ranged from 2.32 to 112.45% and ferric-reducing antioxidant power (FRAP) activity ranging from 21.68 to 179.66 (mg ascorbic acid eq./100 g). Genome-wide association studies (GWAS) were conducted using 222 diverse accessions and 67 K SNPs distributed across all the seven pearl millet chromosomes. Approximately, 218 SNPs were found to be strongly associated with DPPH and FRAP activity at high confidence [–log (p) > 3.0–7.4]. Furthermore, flanking regions of significantly associated SNPs were explored for candidate gene harvesting. This identified 18 candidate genes related to antioxidant pathway genes (flavanone 7-O-beta-glycosyltransferase, GDSL esterase/lipase, glutathione S-transferase) residing within or near the association signal that can be selected for further functional characterization. Patterns of genetic variability and the associated genes reported in this study are useful findings, which would need further validation before their utilization in molecular breeding for high antioxidant-containing pearl millet cultivars. |
topic |
pearl millet [Pennisetum glaucum (L.) R. Br.] antioxidant activity phenolics germplasm genome-wide association study marker-trait associations |
url |
https://www.frontiersin.org/articles/10.3389/fpls.2021.599649/full |
work_keys_str_mv |
AT chandrabhanyadav identifyingantioxidantbiosynthesisgenesinpearlmilletpennisetumglaucumlrbrusinggenomewideassociationanalysis AT jayantitokas identifyingantioxidantbiosynthesisgenesinpearlmilletpennisetumglaucumlrbrusinggenomewideassociationanalysis AT jayantitokas identifyingantioxidantbiosynthesisgenesinpearlmilletpennisetumglaucumlrbrusinggenomewideassociationanalysis AT devvartyadav identifyingantioxidantbiosynthesisgenesinpearlmilletpennisetumglaucumlrbrusinggenomewideassociationanalysis AT devvartyadav identifyingantioxidantbiosynthesisgenesinpearlmilletpennisetumglaucumlrbrusinggenomewideassociationanalysis AT anawinters identifyingantioxidantbiosynthesisgenesinpearlmilletpennisetumglaucumlrbrusinggenomewideassociationanalysis AT rambsingh identifyingantioxidantbiosynthesisgenesinpearlmilletpennisetumglaucumlrbrusinggenomewideassociationanalysis AT ramayadav identifyingantioxidantbiosynthesisgenesinpearlmilletpennisetumglaucumlrbrusinggenomewideassociationanalysis AT prakashigangashetty identifyingantioxidantbiosynthesisgenesinpearlmilletpennisetumglaucumlrbrusinggenomewideassociationanalysis AT rakeshksrivastava identifyingantioxidantbiosynthesisgenesinpearlmilletpennisetumglaucumlrbrusinggenomewideassociationanalysis AT rattansyadav identifyingantioxidantbiosynthesisgenesinpearlmilletpennisetumglaucumlrbrusinggenomewideassociationanalysis |
_version_ |
1721424262744506368 |