Evaluation of relative quantification of alternatively spliced transcripts using droplet digital PCR

Evaluation of relative quantification of alternatively spliced transcripts using droplet digital PCR

Introduction: For the relative quantification of isoform expression, RT-qPCR has been the gold standard for over a decade. More recently, digital PCR is becoming widely implemented, as it is promised to be more accurate, sensitive and less affected by inhibitors, without the need for standard curves...

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Main Authors: Mattias Van Heetvelde, Wouter Van Loocke, Wim Trypsteen, Annelot Baert, Katrien Vanderheyden, Brecht Crombez, Jo Vandesompele, Kim De Leeneer, Kathleen B.M. Claes
Format: Article
Language:English
Published: Elsevier 2017-09-01
Series:Biomolecular Detection and Quantification
Online Access:http://www.sciencedirect.com/science/article/pii/S2214753517301997

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http://www.sciencedirect.com/science/article/pii/S2214753517301997

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