Quantitative Flow Cytometric Evaluation of Oxidative Stress and Mitochondrial Impairment in RAW 264.7 Macrophages after Exposure to Pristine, Acid Functionalized, or Annealed Carbon Nanotubes

Quantitative Flow Cytometric Evaluation of Oxidative Stress and Mitochondrial Impairment in RAW 264.7 Macrophages after Exposure to Pristine, Acid Functionalized, or Annealed Carbon Nanotubes

Conventional nanotoxicological assays are subjected to various interferences with nanoparticles and especially carbon nanotubes. A multiparametric flow cytometry (FCM) methodology was developed here as an alternative to quantify oxidative stress, mitochondrial impairment, and later cytotoxic and gen...

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Bibliographic Details
Main Authors: Odile Sabido, Agathe Figarol, Jean-Philippe Klein, Valérie Bin, Valérie Forest, Jérémie Pourchez, Bice Fubini, Michèle Cottier, Maura Tomatis, Delphine Boudard
Format: Article
Language:English
Published: MDPI AG 2020-02-01
Series:Nanomaterials
Subjects:
carbon nanotubes
acid functionalization
annealing treatment
oxidative stress
mitochondrial membrane potential collapse
cytotoxicity
chromatin decondensation, scavenging capacity
flow cytometry and spin trapping in-cell free system
Online Access:https://www.mdpi.com/2079-4991/10/2/319

Internet

https://www.mdpi.com/2079-4991/10/2/319

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