NormQ: RNASeq normalization based on RT-qPCR derived size factors

NormQ: RNASeq normalization based on RT-qPCR derived size factors

The merit of RNASeq data relies heavily on correct normalization. However, most methods assume that the majority of transcripts show no differential expression between conditions. This assumption may not always be correct, especially when one condition results in overexpression. We present a new met...

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Bibliographic Details
Main Authors: Ravindra Naraine, Pavel Abaffy, Monika Sidova, Silvie Tomankova, Kseniia Pocherniaieva, Ondrej Smolik, Mikael Kubista, Martin Psenicka, Radek Sindelka
Format: Article
Language:English
Published: Elsevier 2020-01-01
Series:Computational and Structural Biotechnology Journal
Subjects:
Transcriptomics
RNASeq
Normalization
TOMOSeq
DESeq
Median-of-ratios
Online Access:http://www.sciencedirect.com/science/article/pii/S2001037020302737

Internet

http://www.sciencedirect.com/science/article/pii/S2001037020302737

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