Rapid Detection of HLA-B*57:01-Expressing Cells Using a Label-Free Interdigitated Electrode Biosensor Platform for Prevention of Abacavir Hypersensitivity in HIV Treatment

Rapid Detection of HLA-B*57:01-Expressing Cells Using a Label-Free Interdigitated Electrode Biosensor Platform for Prevention of Abacavir Hypersensitivity in HIV Treatment

Pre-treatment screening of individuals for human leukocyte antigens (HLA) HLA-B*57:01 is recommended for the prevention of life-threatening hypersensitivity reactions to abacavir, a drug widely prescribed for HIV treatment. However, the implementation of screening in clinical practice is hindered by...

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Main Authors: Jianxiong Chan, Gita V. Soraya, Lauren Craig, Shah M. Uddin, Marian Todaro, Duc H. Huynh, Chathurika D. Abeyrathne, Lyudmila Kostenko, James McCluskey, Efstratios Skafidas, Patrick Kwan
Format: Article
Language:English
Published: MDPI AG 2019-08-01
Series:Sensors
Subjects:
human leukocyte antigen
interdigitated electrodes
impedance sensor
protein sensor
point-of-care diagnostics
pharmacogenetics
Online Access:https://www.mdpi.com/1424-8220/19/16/3543
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spelling doaj-c86b9cc954ef41f1ba52d5aacf8eaca82020-11-24T22:20:48ZengMDPI AGSensors1424-82202019-08-011916354310.3390/s19163543s19163543Rapid Detection of HLA-B*57:01-Expressing Cells Using a Label-Free Interdigitated Electrode Biosensor Platform for Prevention of Abacavir Hypersensitivity in HIV TreatmentJianxiong Chan0Gita V. Soraya1Lauren Craig2Shah M. Uddin3Marian Todaro4Duc H. Huynh5Chathurika D. Abeyrathne6Lyudmila Kostenko7James McCluskey8Efstratios Skafidas9Patrick Kwan10Department of Medicine, Royal Melbourne Hospital, The University of Melbourne, Victoria 3050, AustraliaDepartment of Medicine, Royal Melbourne Hospital, The University of Melbourne, Victoria 3050, AustraliaDepartment of Medicine, Royal Melbourne Hospital, The University of Melbourne, Victoria 3050, AustraliaDepartment of Medicine, Royal Melbourne Hospital, The University of Melbourne, Victoria 3050, AustraliaDepartment of Medicine, Royal Melbourne Hospital, The University of Melbourne, Victoria 3050, AustraliaDepartment of Electrical and Electronic Engineering, Melbourne School of Engineering, The University of Melbourne, Victoria 3010, AustraliaDepartment of Electrical and Electronic Engineering, Melbourne School of Engineering, The University of Melbourne, Victoria 3010, AustraliaDepartment of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Victoria 3010, AustraliaDepartment of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Victoria 3010, AustraliaDepartment of Electrical and Electronic Engineering, Melbourne School of Engineering, The University of Melbourne, Victoria 3010, AustraliaDepartment of Medicine, Royal Melbourne Hospital, The University of Melbourne, Victoria 3050, AustraliaPre-treatment screening of individuals for human leukocyte antigens (HLA) HLA-B*57:01 is recommended for the prevention of life-threatening hypersensitivity reactions to abacavir, a drug widely prescribed for HIV treatment. However, the implementation of screening in clinical practice is hindered by the slow turnaround time and high cost of conventional HLA genotyping methods. We have developed a biosensor platform using interdigitated electrode (IDE) functionalized with a monoclonal antibody to detect cells expressing HLA-B*57:01. This platform was evaluated using cell lines and peripheral blood mononuclear cells expressing different HLA-B alleles. The functionalized IDE sensor was able to specifically capture HLA-B*57:01 cells, resulting in a significant change in the impedance magnitude in 20 min. This IDE platform has the potential to be further developed to enable point-of-care HLA-B*57:01 screening.https://www.mdpi.com/1424-8220/19/16/3543human leukocyte antigeninterdigitated electrodesimpedance sensorprotein sensorpoint-of-care diagnosticspharmacogenetics
collection DOAJ
language English
format Article
sources DOAJ
author Jianxiong Chan
Gita V. Soraya
Lauren Craig
Shah M. Uddin
Marian Todaro
Duc H. Huynh
Chathurika D. Abeyrathne
Lyudmila Kostenko
James McCluskey
Efstratios Skafidas
Patrick Kwan
spellingShingle Jianxiong Chan
Gita V. Soraya
Lauren Craig
Shah M. Uddin
Marian Todaro
Duc H. Huynh
Chathurika D. Abeyrathne
Lyudmila Kostenko
James McCluskey
Efstratios Skafidas
Patrick Kwan
Rapid Detection of HLA-B*57:01-Expressing Cells Using a Label-Free Interdigitated Electrode Biosensor Platform for Prevention of Abacavir Hypersensitivity in HIV Treatment
Sensors
human leukocyte antigen
interdigitated electrodes
impedance sensor
protein sensor
point-of-care diagnostics
pharmacogenetics
author_facet Jianxiong Chan
Gita V. Soraya
Lauren Craig
Shah M. Uddin
Marian Todaro
Duc H. Huynh
Chathurika D. Abeyrathne
Lyudmila Kostenko
James McCluskey
Efstratios Skafidas
Patrick Kwan
author_sort Jianxiong Chan
title Rapid Detection of HLA-B*57:01-Expressing Cells Using a Label-Free Interdigitated Electrode Biosensor Platform for Prevention of Abacavir Hypersensitivity in HIV Treatment
title_short Rapid Detection of HLA-B*57:01-Expressing Cells Using a Label-Free Interdigitated Electrode Biosensor Platform for Prevention of Abacavir Hypersensitivity in HIV Treatment
title_full Rapid Detection of HLA-B*57:01-Expressing Cells Using a Label-Free Interdigitated Electrode Biosensor Platform for Prevention of Abacavir Hypersensitivity in HIV Treatment
title_fullStr Rapid Detection of HLA-B*57:01-Expressing Cells Using a Label-Free Interdigitated Electrode Biosensor Platform for Prevention of Abacavir Hypersensitivity in HIV Treatment
title_full_unstemmed Rapid Detection of HLA-B*57:01-Expressing Cells Using a Label-Free Interdigitated Electrode Biosensor Platform for Prevention of Abacavir Hypersensitivity in HIV Treatment
title_sort rapid detection of hla-b*57:01-expressing cells using a label-free interdigitated electrode biosensor platform for prevention of abacavir hypersensitivity in hiv treatment
publisher MDPI AG
series Sensors
issn 1424-8220
publishDate 2019-08-01
description Pre-treatment screening of individuals for human leukocyte antigens (HLA) HLA-B*57:01 is recommended for the prevention of life-threatening hypersensitivity reactions to abacavir, a drug widely prescribed for HIV treatment. However, the implementation of screening in clinical practice is hindered by the slow turnaround time and high cost of conventional HLA genotyping methods. We have developed a biosensor platform using interdigitated electrode (IDE) functionalized with a monoclonal antibody to detect cells expressing HLA-B*57:01. This platform was evaluated using cell lines and peripheral blood mononuclear cells expressing different HLA-B alleles. The functionalized IDE sensor was able to specifically capture HLA-B*57:01 cells, resulting in a significant change in the impedance magnitude in 20 min. This IDE platform has the potential to be further developed to enable point-of-care HLA-B*57:01 screening.
topic human leukocyte antigen
interdigitated electrodes
impedance sensor
protein sensor
point-of-care diagnostics
pharmacogenetics
url https://www.mdpi.com/1424-8220/19/16/3543
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