Increased osteoblast viability at alkaline pH in vitro provides a new perspective on bone regeneration

We investigated the effects of alkaline pH on developing osteoblasts. Cells of the osteoblast-like cell line MC3T3-E1 were initially cultured for six days in HEPES-buffered media with pH ranging from 7.2 to 9.0. Cell count, cellular WST-1 metabolism, and ATP content were analyzed. The three paramete...

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Bibliographic Details
Main Authors: Anne-Marie Galow, Alexander Rebl, Dirk Koczan, Sebastian M. Bonk, Werner Baumann, Jan Gimsa
Format: Article
Language:English
Published: Elsevier 2017-07-01
Series:Biochemistry and Biophysics Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2405580816303429