Increased osteoblast viability at alkaline pH in vitro provides a new perspective on bone regeneration
We investigated the effects of alkaline pH on developing osteoblasts. Cells of the osteoblast-like cell line MC3T3-E1 were initially cultured for six days in HEPES-buffered media with pH ranging from 7.2 to 9.0. Cell count, cellular WST-1 metabolism, and ATP content were analyzed. The three paramete...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2017-07-01
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Series: | Biochemistry and Biophysics Reports |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580816303429 |