Combined DECS Analysis and Next-Generation Sequencing Enable Efficient Detection of Novel Plant RNA Viruses

Combined DECS Analysis and Next-Generation Sequencing Enable Efficient Detection of Novel Plant RNA Viruses

The presence of high molecular weight double-stranded RNA (dsRNA) within plant cells is an indicator of infection with RNA viruses as these possess genomic or replicative dsRNA. DECS (dsRNA isolation, exhaustive amplification, cloning, and sequencing) analysis has been shown to be capable of detecti...

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Bibliographic Details
Main Authors: Hironobu Yanagisawa, Reiko Tomita, Koji Katsu, Takuya Uehara, Go Atsumi, Chika Tateda, Kappei Kobayashi, Ken-Taro Sekine
Format: Article
Language:English
Published: MDPI AG 2016-03-01
Series:Viruses
Subjects:
deep-sequencing
dsRNA
dsRNA-binding protein
detection
blueberry
Sobemovirus
Blueberry shoestring virus
Online Access:http://www.mdpi.com/1999-4915/8/3/70

Internet

http://www.mdpi.com/1999-4915/8/3/70

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