Chemical clearing and dehydration of GFP expressing mouse brains.

Generally, chemical tissue clearing is performed by a solution consisting of two parts benzyl benzoate and one part benzyl alcohol. However, prolonged exposure to this mixture markedly reduces the fluorescence of GFP expressing specimens, so that one has to compromise between clearing quality and fl...

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Bibliographic Details
Main Authors: Klaus Becker, Nina Jährling, Saiedeh Saghafi, Reto Weiler, Hans-Ulrich Dodt
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22479475/?tool=EBI