Dengue Virus Directly Stimulates Polyclonal B Cell Activation.
Dengue infection is associated to vigorous inflammatory response, to a high frequency of activated B cells, and to increased levels of circulating cross-reactive antibodies. We investigated whether direct infection of B cells would promote activation by culturing primary human B lymphocytes from hea...
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doaj-c8f36b11200644f7a50a7d7c159ae53f2020-11-25T01:22:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011012e014339110.1371/journal.pone.0143391Dengue Virus Directly Stimulates Polyclonal B Cell Activation.Arturo Ramon Vargas CorreaAna Carolina Egypto Rosa BerbelMichelle Premazzi PapaAna Theresa Silveira de MoraisLigia Maria Torres PeçanhaLuciana Barros de ArrudaDengue infection is associated to vigorous inflammatory response, to a high frequency of activated B cells, and to increased levels of circulating cross-reactive antibodies. We investigated whether direct infection of B cells would promote activation by culturing primary human B lymphocytes from healthy donors with DENV in vitro. B cells were susceptible, but poorly permissive to infection. Even though, primary B cells cultured with DENV induced substantial IgM secretion, which is a hallmark of polyclonal B cell activation. Notably, DENV induced the activation of B cells obtained from either DENV immune or DENV naïve donors, suggesting that it was not dependent on DENV-specific secondary/memory response. B cell stimulation was dependent on activation of MAPK and CD81. B cells cultured with DENV also secreted IL-6 and presented increased expression of CD86 and HLA-DR, which might contribute to B lymphocyte co-stimulatory function. Indeed, PBMCs, but not isolated B cells, secreted high amounts of IgG upon DENV culture, suggesting that interaction with other cell types in vivo might promote Ig isotype switching and IgG secretion from different B cell clones. These findings suggest that activation signaling pathways triggered by DENV interaction with non-specific receptors on B cells might contribute to the exacerbated response observed in dengue patients.http://europepmc.org/articles/PMC4675537?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Arturo Ramon Vargas Correa Ana Carolina Egypto Rosa Berbel Michelle Premazzi Papa Ana Theresa Silveira de Morais Ligia Maria Torres Peçanha Luciana Barros de Arruda |
spellingShingle |
Arturo Ramon Vargas Correa Ana Carolina Egypto Rosa Berbel Michelle Premazzi Papa Ana Theresa Silveira de Morais Ligia Maria Torres Peçanha Luciana Barros de Arruda Dengue Virus Directly Stimulates Polyclonal B Cell Activation. PLoS ONE |
author_facet |
Arturo Ramon Vargas Correa Ana Carolina Egypto Rosa Berbel Michelle Premazzi Papa Ana Theresa Silveira de Morais Ligia Maria Torres Peçanha Luciana Barros de Arruda |
author_sort |
Arturo Ramon Vargas Correa |
title |
Dengue Virus Directly Stimulates Polyclonal B Cell Activation. |
title_short |
Dengue Virus Directly Stimulates Polyclonal B Cell Activation. |
title_full |
Dengue Virus Directly Stimulates Polyclonal B Cell Activation. |
title_fullStr |
Dengue Virus Directly Stimulates Polyclonal B Cell Activation. |
title_full_unstemmed |
Dengue Virus Directly Stimulates Polyclonal B Cell Activation. |
title_sort |
dengue virus directly stimulates polyclonal b cell activation. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
Dengue infection is associated to vigorous inflammatory response, to a high frequency of activated B cells, and to increased levels of circulating cross-reactive antibodies. We investigated whether direct infection of B cells would promote activation by culturing primary human B lymphocytes from healthy donors with DENV in vitro. B cells were susceptible, but poorly permissive to infection. Even though, primary B cells cultured with DENV induced substantial IgM secretion, which is a hallmark of polyclonal B cell activation. Notably, DENV induced the activation of B cells obtained from either DENV immune or DENV naïve donors, suggesting that it was not dependent on DENV-specific secondary/memory response. B cell stimulation was dependent on activation of MAPK and CD81. B cells cultured with DENV also secreted IL-6 and presented increased expression of CD86 and HLA-DR, which might contribute to B lymphocyte co-stimulatory function. Indeed, PBMCs, but not isolated B cells, secreted high amounts of IgG upon DENV culture, suggesting that interaction with other cell types in vivo might promote Ig isotype switching and IgG secretion from different B cell clones. These findings suggest that activation signaling pathways triggered by DENV interaction with non-specific receptors on B cells might contribute to the exacerbated response observed in dengue patients. |
url |
http://europepmc.org/articles/PMC4675537?pdf=render |
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