Post-translational Regulation of Cas9 during G1 Enhances Homology-Directed Repair

Post-translational Regulation of Cas9 during G1 Enhances Homology-Directed Repair

CRISPR/Cas9 induces DNA double-strand breaks that are repaired by cell-autonomous repair pathways, namely, non-homologous end-joining (NHEJ), or homology-directed repair (HDR). While HDR is absent in G1, NHEJ is active throughout the cell cycle and, thus, is largely favored over HDR. We devised a st...

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Bibliographic Details
Main Authors: Tony Gutschner, Monika Haemmerle, Giannicola Genovese, Giulio F. Draetta, Lynda Chin
Format: Article
Language:English
Published: Elsevier 2016-02-01
Series:Cell Reports
Subjects:
CRISPR
cell cycle
genome editing
MALAT1
homologous recombination
proteolysis
synthetic biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124716000401

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http://www.sciencedirect.com/science/article/pii/S2211124716000401

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