FACS-based isolation of fixed mouse neuronal nuclei for ATAC-seq and Hi-C

FACS-based isolation of fixed mouse neuronal nuclei for ATAC-seq and Hi-C

Summary: The organization of chromatin structure plays a crucial role in gene expression, DNA replication, and repair. Chromatin alterations influence gene expression, and modifications could be associated with genomic instability in the cells during aging or diseases. Here, we provide a modified pr...

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Bibliographic Details
Main Authors: Ekaterina Eremenko, Anastasia Golova, Daniel Stein, Monica Einav, Ekaterina Khrameeva, Debra Toiber
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Flow Cytometry/Mass Cytometry
Cell separation/fractionation
Genomics
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721003506

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http://www.sciencedirect.com/science/article/pii/S2666166721003506

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