The impact of different DNA extraction methods on the analysis of microbial diversity of oral saliva from healthy youths by polymerase chain reaction-denaturing gradient gel electrophoresis

Background/purpose: Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), as a conventional molecular technique, was utilized to analyze the diversity of oral microbiota. However, studies found that the results of PCR-DGGE were affected by the DNA isolation method. This study...

Full description

Bibliographic Details
Main Authors: Ming Chen, Bu-Ling Wu, Ting Chen, Zhao Liu, Zi-Long Deng, Ling Peng
Format: Article
Language:English
Published: Elsevier 2016-03-01
Series:Journal of Dental Sciences
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1991790215000975