Improving heterologous membrane protein production in Escherichia coli by combining transcriptional tuning and codon usage algorithms.

Improving heterologous membrane protein production in Escherichia coli by combining transcriptional tuning and codon usage algorithms.

High-level, recombinant production of membrane-integrated proteins in Escherichia coli is extremely relevant for many purposes, but has also been proven challenging. Here we study a combination of transcriptional fine-tuning in E. coli LEMO21(DE3) with different codon usage algorithms for heterologo...

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Bibliographic Details
Main Authors: Nico J Claassens, Melvin F Siliakus, Sebastiaan K Spaans, Sjoerd C A Creutzburg, Bart Nijsse, Peter J Schaap, Tessa E F Quax, John van der Oost
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5597330?pdf=render

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http://europepmc.org/articles/PMC5597330?pdf=render

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