Improvement of solubility and refolding of an anti-human epidermal growth factor receptor 2 single-chain antibody fragment inclusion bodies

Improvement of solubility and refolding of an anti-human epidermal growth factor receptor 2 single-chain antibody fragment inclusion bodies

Single chain variable fragment antibodies (scFvs) have attracted many attentions due to their small size, faster bio-distribution and better penetration in to the target tissues, and ease of expression in Escherichia coli. Although, scFv expression in E. coli usually leads to formation of inclusion...

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Bibliographic Details
Main Authors: Javad Salehinia, Hamid Mir Mohammad Sadeghi, Daryoush Abedi, Vajihe Akbari
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2018-01-01
Series:Research in Pharmaceutical Sciences
Subjects:
her2; inclusion bodies; refolding additive; response surface methodology; single-chain antibodies; solubilizing
Online Access:http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2018;volume=13;issue=6;spage=566;epage=574;aulast=Salehinia

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http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2018;volume=13;issue=6;spage=566;epage=574;aulast=Salehinia

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