Controlled Growth and the Maintenance of Human Pluripotent Stem Cells by Cultivation with Defined Medium on Extracellular Matrix-Coated Micropatterned Dishes.

Controlled Growth and the Maintenance of Human Pluripotent Stem Cells by Cultivation with Defined Medium on Extracellular Matrix-Coated Micropatterned Dishes.

Here, we introduce a new serum-free defined medium (SPM) that supports the cultivation of human pluripotent stem cells (hPSCs) on recombinant human vitronectin-N (rhVNT-N)-coated dishes after seeding with either cell clumps or single cells. With this system, there was no need for an intervening sequ...

Full description

Bibliographic Details
Main Authors: Chiemi Takenaka, Hiroshi Miyajima, Yusuke Yoda, Hideo Imazato, Takako Yamamoto, Shinichi Gomi, Yasuhiro Ohshima, Kenichi Kagawa, Tetsuji Sasaki, Shin Kawamata
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0129855

Internet

https://doi.org/10.1371/journal.pone.0129855

Similar Items